Human PRAS40 Antibody

Detection of Human PRAS40 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, A549 human lung carcinoma cell line, MDA-MB-468 human breast cancer cell line, and T47D human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human PRAS40 Monoclonal Antibody (Catalog # MAB6408) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PRAS40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human PRAS40 by Simple Western^TM. Simple Western lane view shows lysates of 293T human embryonic kidney cell line, loaded at 0.5 mg/mL. A specific band was detected for PRAS40 at approximately 65 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human PRAS40 Monoclonal Antibody (Catalog # MAB6408). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

PRAS40 in HeLa Human Cell Line. PRAS40 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human PRAS40 Monoclonal Antibody (Catalog # MAB6408) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.