Human PON2 Antibody Summary
Ala30-Leu354
Accession # Q15165
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human PON2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human PON2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4344) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PON2 at approximately 42-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PON2
The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7. Paraoxonase/arylesterase 2 (PON2) is a 354 amino acid, 39 kDa protein that is widely expressed in a variety of tissues and may act as a cellular antioxidant, protecting cells from oxidative stress. PON2 has arylesterase and aryldialkylphosphatase activity (EC 3.1.1.2 and EC 3.1.8.1) and can hydrolyze a number of organophosphate substrates and aromatic carboxylic acid esters. PON2 is membrane-bound and has several potential glycosylation sites. Sequence polymorphisms in this gene may be associated with coronary heart disease and a number of phenotypes related to diabetes. PON2 is not associated with HDL but can prevent LDL lipid peroxidation and reverse the oxidation of mildly oxidized LDL. Alternatively spliced transcript variants encoding different isoforms have been described.
Product Datasheets
Citations for Human PON2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Variation among intact tissue samples reveals the core transcriptional features of human CNS cell classes
Authors: Kevin W. Kelley, Hiromi Nakao-Inoue, Anna V. Molofsky, Michael C. Oldham
Nature Neuroscience
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Paraoxonase 2 overexpression inhibits tumor development in a mouse model of ovarian cancer
Authors: A Devarajan, F Su, V Grijalva, M Yalamanchi, A Yalamanchi, F Gao, H Trost, J Nwokedi, G Farias-Eis, R Farias-Eis, AM Fogelman, ST Reddy
Cell Death Dis, 2018-03-12;9(3):392.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
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