Human Polypeptide GalNAc Transferase 4/GALNT4 Antibody
Human Polypeptide GalNAc Transferase 4/GALNT4 Antibody Summary
Ala36-Lys578
Accession # Q8N4A0
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse Polypeptide GalNac Transferase 4/GALNT4 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and mouse liver tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Polypeptide GalNac Transferase 4/GALNT4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7528) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Polypeptide GalNac Transferase 4/GALNT4 at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Polypeptide GalNAc Transferase 4/GALNT4
GALNT4 (Polypeptide N‑acetylgalactosaminyltransferase 4; also Polypeptide GalNAc transferase 4 and UDP-GalNAc:polypeptide Nacetylgalactosaminyltransferase 4) is a 66 kDa member of the GalNAC transferase subfamily, glycosyltransferase 4 family of enzymes. It is widely expressed, highly expressed in mucous cells. GALNT4 is found in the Golgi apparatus, and catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Human GALNT4 is a 578 amino acid (aa) type II transmembrane protein. It contains a twelve aa N-terminal cytoplasmic region and a 543 aa extracellular lumenal domain (aa 36-578). There is a potential 45 kDa splice form variant. Over aa 36-578, human GALNT4 shares 92% aa sequence identity with mouse GALNT4.
Product Datasheets
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