Human Plexin A1 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB6536N Datasheet

Catalog #	Availability	Size / Price	Qty
FAB6536N-100UG

All Plexin A1 Antibodies

Product Details

FAQs

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Plexin A1 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Plexin A1 in direct ELISAs. In Western blots, no cross-reactivity with recombinant human Plexin A4, B1, B2, B3, C1, D1, recombinant mouse Plexin A1, A2, or A3 is observed.

Source

Monoclonal Mouse IgG1 Clone # 659807

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Plexin A1
Asn986-Leu1152
Accession # Q9UIW2

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Plexin A1

Plexin A1 is an approximately 200 kDa type I transmembrane member of the Plexin family of Semaphorin signal transducers. It consists of a 1218 amino acid (aa) extracellular domain with one Sema domain, a spacer, and four tandem IPT/TIG domains, a 21 aa transmembrane segment, and a 631 aa cytoplasmic domain. Plexin A1 binds the transmembrane Semaphorin 6D directly and binds the Class 3 secreted Semaphorins indirectly via Neuropilin-1 and -2. It mediates cytoskeletal remodeling that results in cell migration and axon repulsion. Within aa 964-1152 (in the IPT/TIG region), human Plexin A1 shares 93% aa sequence identity with mouse and rat Plexin A1.

Entrez Gene IDs

5361 (Human); 18844 (Mouse)

Alternate Names

NOV; NOVP; Plexin A1; PLEXIN-A1; PLXN1; PLXNA1