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Human PIKFyve Antibody

Catalog #: AF7885
3 Citations Datasheet
Catalog # Availability Size / Price Qty
AF7885
AF7885-SP
Detection of Human PIKFyve by Western Blot.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PIKFyve Antibody Summary

Species Reactivity
Human
Specificity
Detects human PIKFyve in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PIKFyve
Lys215-Lys361
Accession # Q9Y2I7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PIKFyve antibody by Western Blot. View Larger

Detection of Human PIKFyve by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human PIKFyve Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7885) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PIKFyve at approximately 240 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PIKFyve

PIKfyve (PhosphoInositide Kinase for five position containing a fyve finger (FAB1/YOTB/Vac1/EEA1) domain; also phosphatidylinositol 3-phosphate 5-kinase type III and Type III PIP kinase) is a 230-260 kDa cytoplasmic and endocytic member of an ancient family of phosphatidylinositol-3,5 biphosphate (PI[3,5]P2 synthesizing enzymes. It is associated with endosomes, particularly the microdomains of early endosomes that are EEA1-deficient. PIKfyve binds to membrane-embedded phosphatidylinositol and initially converts PI3P into PI(3,5)P2, which is then converted into PI5P. In conjunction with ArPIKfyve and Sac3, its actions are involved in the normal transport, fusion, and membrane export of endosomes. Notably, PIKfyve also appears to act as a protein kinase, apparently controlling its own activity through autophosphorylation. Human PIKfyve is 2098 amino acids (aa) in length. It contains one FYVE PI3P-binding zinc-finger domain (aa 154-219), a DEP domain (aa 365-440) and a C-terminal PIP kinase rehion (aa 1172-2085). There are no less than 29 utilized Ser/Thr phosphorylation sites, plus two utilized Tyr phosphorylation sites. Three potential isoform variants are reported, all which possess a three aa substitution for aa 546-2098. Two of these three also demonstrate a deletion of aa 108-204, and a nine aa substitution for aa 109-203, respectively. Over aa 215-361, human and mouse share 95% aa sequence identity.

Long Name
Phosphoinositide Kinase, FYVE Finger-containing
Entrez Gene IDs
200576 (Human); 18711 (Mouse); 316457 (Rat)
Alternate Names
1-phosphatidylinositol-3-phosphate 5-kinase; EC 2.7.1.150; FAB1; FLJ37746; FYVE finger-containing phosphoinositide kinase; KIAA0981CFD; MGC40423; p235; Phosphatidylinositol-3-phosphate 5-kinase type III; Phosphatidylinositol-3-phosphate 5-kinase; phosphoinositide kinase, FYVE finger containing; PIKFyve; PIP5K; PIP5K3; PIP5K3FAB1; PIPkin-III; Type III PIP kinase; type III

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Citations for Human PIKFyve Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Rab GTPases and phosphoinositides fine-tune SNAREs dependent targeting specificity of intracellular vesicle traffic
    Authors: Koike, S;Jahn, R;
    Nature communications
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Trehalose limits opportunistic mycobacterial survival during HIV co-infection by reversing HIV-mediated autophagy block
    Authors: V Sharma, M Makhdoomi, L Singh, P Kumar, N Khan, S Singh, HN Verma, K Luthra, S Sarkar, D Kumar
    Autophagy, 2020-02-20;0(0):1-20.
    Species: Human
    Sample Types:
  3. Autophagy inhibition by targeting PIKfyve potentiates response to immune checkpoint blockade in prostate cancer
    Authors: Yuanyuan Qiao, Jae Eun Choi, Jean C. Tien, Stephanie A. Simko, Thekkelnaycke Rajendiran, Josh N. Vo et al.
    Nature Cancer

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