Human Phospho-STAT3 (Y705) Antibody

Catalog #: MAB4607
5 Citations Datasheet

Catalog #	Availability	Size / Price	Qty
MAB4607
MAB4607-SP

Detection of Human Phospho‑STAT3 (Y705) by Western Blot.

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Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-STAT3 (Y705) Antibody Summary

Species Reactivity

Human

Specificity

Detects human STAT3 when phosphorylated at Y705.

Source

Monoclonal Rat IgG_2B Clone # 805435

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Phosphopeptide containing the human STAT3 Y705 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho‑STAT3 (Y705) by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes or 50 µg/mL Recombinant Human IL-6 (Catalog # 206-IL) for 15 minutes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Phospho-STAT3 (Y705) Monoclonal Antibody (Catalog # MAB4607) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-STAT3 (Y705) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT3

Human STAT-3/STAT3a (signal transducer and activator of transcription 3; also called APRF) is a 90-95 kDa member of the STAT family of transcription factors. It is 770 amino acids (aa) in length, contains one SH2 domain (aa 580-670), and is found in almost all cell types. STAT3 mediates gp130, LIF-R, OB-R, IL10-R and EGFR signaling. Upon activation, receptors such as gp130 phosphorylate STAT3 at Ser727, which results in transcriptional activation. Phosphorylation at Tyr705 mediates homo- and hetero-dimerization with STAT1 and nuclear translocation, and is an important event in oncogenic transformation. The STAT3b isoform shows a six aa substitution for aa 716-770, thus eliminating the Ser727 site. Human and mouse STAT3a share 99% aa sequence identity.

Long Name

Signal Transducer and Activator of Transcription 3

Entrez Gene IDs

6774 (Human); 20848 (Mouse); 25125 (Rat)

Alternate Names

Acute-phase response factor; APRFMGC16063; DNA-binding protein APRF; FLJ20882; HIES; signal transducer and activator of transcription 3 (acute-phase responsefactor); signal transducer and activator of transcription 3; STAT3

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human Phospho-STAT3 (Y705) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Effects of Tofacitinib on Muscle Remodeling in Experimental Rheumatoid Sarcopenia
Authors: Bermejo-Álvarez, I;Pérez-Baos, S;Gratal, P;Medina, JP;Largo, R;Herrero-Beaumont, G;Mediero, A;
International journal of molecular sciences
Species: Rabbit
Sample Types: Tissue Homogenates
Applications: Western Blot
Thymosin ?4 Alleviates Autoimmune Dacryoadenitis via Suppressing Th17 Cell Response
Authors: Zhao, X;Li, N;Yang, N;Mi, B;Dang, W;Sun, D;Ma, S;Nian, H;Wei, R;
Investigative ophthalmology & visual science
Species: Rabbit
Sample Types: Cell Lysates
Applications: Western Blot
Tumor-derived mesenchymal-stem-cell-secreted IL-6 enhances resistance to cisplatin via the STAT3 pathway in breast cancer
Authors: H Xu, Y Zhou, W Li, B Zhang, H Zhang, S Zhao, P Zheng, H Wu, J Yang
Oncol Lett, 2018-04-11;15(6):9142-9150.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis
Authors: RD Little, I Prieto-Pot, S Pérez-Baos, A Villalvill, P Gratal, F Cicuttini, R Largo, G Herrero-Be
Sci Rep, 2017-07-24;7(1):6311.
Species: Rabbit
Sample Types: Tissue Homogenates
Applications: Western Blot
Inhibition of pSTAT1 by tofacitinib accounts for the early improvement of experimental chronic synovitis
Authors: Sandra Pérez-Baos, Paula Gratal, Juan I. Barrasa, Ana Lamuedra, Olga Sánchez-Pernaute, Gabriel Herrero-Beaumont et al.
Journal of Inflammation