Human Phospho-STAT3 (Y705) Antibody

Catalog #: MAB46071
2 Citations Datasheet

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB46071-100
MAB46071-SP

Detection of Human Phospho-STAT3 (Y705) by Western Blot.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-STAT3 (Y705) Antibody Summary

Species Reactivity

Human

Specificity

Detects human STAT3 when phosphorylated at Y705 in Western blots.

Source

Recombinant Monoclonal Rabbit IgG_2B Clone # 1004G

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Phosphopeptide containing the human STAT3 Y705 site
Accession # P40763

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Simple Western

2 µg/mL

Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 50 µg/ml Recombinant Human IL-22 (Catalog # 782-IL) for 15 minutes

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-STAT3 (Y705) by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-22 (Catalog # 782-IL) for 15 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-STAT3 (Y705) Monoclonal Antibody (Catalog # MAB46071) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT3 (Y705) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry

Detection of Phospho-STAT3 (Y705) antibody in IFN alpha-treated Daudi Human Cell Line antibody by Flow Cytometry.

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Detection of Phospho-STAT3 (Y705) in IFN alpha-treated Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was unstimulated (open histogram) or treated with 500 U/mL rhIFN-alpha for 20 minutes (filled histogram) and stained with Rabbit anti-Human Phospho-STAT3 (Y705) Molyclonal Antibody (Catalog # MAB46071) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

Simple Western

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Detection of Human STAT3 by Simple Western^TM. Simple Western shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 50 µg/ml Recombinant Human IL‑22 (Catalog # 782-IL) for 15 minutes, loaded at 0.2 mg/ml. A specific band was detected for STAT3 at approximately 89 kDa (as indicated) using 2 µg/mL of Rabbit Anti-Human Phospho-STAT3 (Y705) Monoclonal Antibody (Catalog # MAB46071). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT3

Human STAT-3/STAT3a (signal transducer and activator of transcription 3; also called APRF) is a 90-95 kDa member of the STAT family of transcription factors. It is 770 amino acids (aa) in length, contains one SH2 domain (aa 580-670), and is found in almost all cell types. STAT3 mediates gp130, LIF-R, OB-R, IL10-R and EGFR signaling. Upon activation, receptors such as gp130 phosphorylate STAT3 at Ser727, which results in transcriptional activation. Phosphorylation at Tyr705 mediates homo- and hetero-dimerization with STAT1 and nuclear translocation, and is an important event in oncogenic transformation. The STAT3b isoform shows a six aa substitution for aa 716-770, thus eliminating the Ser727 site. Human and mouse STAT3a share 99% aa sequence identity.

Long Name

Signal Transducer and Activator of Transcription 3

Entrez Gene IDs

6774 (Human); 20848 (Mouse); 25125 (Rat)

Alternate Names

Acute-phase response factor; APRFMGC16063; DNA-binding protein APRF; FLJ20882; HIES; signal transducer and activator of transcription 3 (acute-phase responsefactor); signal transducer and activator of transcription 3; STAT3

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human Phospho-STAT3 (Y705) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Human omental adipose-derived mesenchymal stem cells enhance autophagy in ovarian carcinoma cells through the STAT3 signalling pathway
Authors: Y Chu, Y Wang, K Li, M Liu, Y Zhang, Y Li, X Hu, C Liu, H Zhou, J Zuo, W Peng
Cell. Signal., 2020-01-24;69(0):109549.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Adipose-derived mesenchymal stem cells promote osteosarcoma proliferation and metastasis by activating the STAT3 pathway
Authors: Y Wang, Y Chu, B Yue, X Ma, G Zhang, H Xiang, Y Liu, T Wang, X Wu, B Chen
Oncotarget, 2017-04-04;8(14):23803-23816.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot