Human Phospho-STAT3 (Y705) Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB4607T Datasheet

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FAB4607T-100UG

All STAT3 Antibodies

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Human Phospho-STAT3 (Y705) Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human STAT3 when phosphorylated at Y705.

Source

Monoclonal Rat IgG2b Clone # 805435

Purification

Protein A or G purified

Immunogen

Phosphopeptide containing the human STAT3 Y705 site

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: STAT3

Human STAT-3/STAT3a (signal transducer and activator of transcription 3; also called APRF) is a 90-95 kDa member of the STAT family of transcription factors. It is 770 amino acids (aa) in length, contains one SH2 domain (aa 580-670), and is found in almost all cell types. STAT3 mediates gp130, LIF-R, OB-R, IL10-R and EGFR signaling. Upon activation, receptors such as gp130 phosphorylate STAT3 at Ser727, which results in transcriptional activation. Phosphorylation at Tyr705 mediates homo- and hetero-dimerization with STAT1 and nuclear translocation, and is an important event in oncogenic transformation. The STAT3b isoform shows a six aa substitution for aa 716-770, thus eliminating the Ser727 site. Human and mouse STAT3a share 99% aa sequence identity.

Long Name

Signal Transducer and Activator of Transcription 3

Entrez Gene IDs

6774 (Human); 20848 (Mouse); 25125 (Rat)

Alternate Names

Acute-phase response factor; APRFMGC16063; DNA-binding protein APRF; FLJ20882; HIES; signal transducer and activator of transcription 3 (acute-phase responsefactor); signal transducer and activator of transcription 3; STAT3