Human Phospho-STAT3 (S727) Antibody

Catalog #: AF4934
1 Citation Datasheet

Catalog #	Availability	Size / Price	Qty
AF4934
AF4934-SP

Detection of Human Phospho-STAT3 (S727) by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-STAT3 (S727) Antibody Summary

Species Reactivity

Human

Specificity

Detects human STAT3 when phosphorylated at S727 in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human STAT3 S727 site
Accession # P40763

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-STAT3 (S727) by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) for 15 minutes. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-STAT3 (S727) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4934) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-STAT3 (S727) was detected at approximately 110 kDa (as indicated). For additional reference, duplicate lysates were probed with 1.0 µg/mL Human STAT3 Monoclonal Antibody (lower panel, Catalog # MAB1799). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT3

PI16 (Peptidase Inhibitor 16) is a variable molecular weight (MW) member of the CRISP family of proteins. It is expressed by cardiomyocytes and serves as an autocrine negative growth regulator. Mature mouse PI16 is a type I transmembrane protein 469 amino acids (aa) in length. The extracellular region includes all but the C‑terminal 21 amino acids. A soluble form of PI16 is known. Although its predicted MW is 50 kDa, it runs anomalously at 70 kDa (unmodified) in SDS‑PAGE. With glycosylation, it appears to be 100‑110 kDa in size. Three potential variants exist. One shows a deletion of aa 51‑124, a second shows a 9 aa extension at the N‑terminus, and a third shows a deletion of aa 191‑452. Over aa 1‑464, mouse PI16 shares 86% and 61% aa identity with rat and human PI16, respectively.

Long Name

Signal Transducer and Activator of Transcription 3

Entrez Gene IDs

6774 (Human); 20848 (Mouse); 25125 (Rat)

Alternate Names

Acute-phase response factor; APRFMGC16063; DNA-binding protein APRF; FLJ20882; HIES; signal transducer and activator of transcription 3 (acute-phase responsefactor); signal transducer and activator of transcription 3; STAT3

Product Datasheets

Product Datasheet

Related Research Areas

Citation for Human Phospho-STAT3 (S727) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation
Authors: J Saarimäki-, D Balboa, MA Russell, J Saarikettu, M Kinnunen, S Keskitalo, A Malhi, C Valensisi, C Andrus, S Eurola, H Grym, J Ustinov, K Wartiovaar, RD Hawkins, O Silvennoin, M Varjosalo, NG Morgan, T Otonkoski
Cell Rep, 2017-04-11;19(2):281-294.
Species: Human
Sample Types:
Applications: Western Blot