Human Phospho-Smad2/3 (S465/S467) Alexa Fluor® 647-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Smad3

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4); and the inhibitory Smads, (Smads 6 and 7). The binding of TGF-beta or activin to their cognate receptor induces phosphorylation of Smads 2 and 3. The activated Smads associate with the common-mediator subunit, Smad4, and the heteromeric complex translocates into the nucleus to initiate transcription. Smad3, also known as Mothers Against Decapentaplegic homolog 3 (MADH3), shares 83% amino acid identity with Smad2, also known as Mothers Against Decapentaplegic homolog 2 (MADH2). Human Smad2 has 99% identity to mouse and rat Smad2. Human Smad3 has 99% identity to mouse and rat Smad3.

Product Datasheets

Related Research Areas

• Airway Inflammation and Remodeling
• Cancer Stem Cell Transcription Factors
• Checkpoint
• EMT Induction
• Intracellular Signaling Molecules in Angiogenesis
• Mesenchymal Stem Cell Transcription Factors
• Neural Stem Cell Transcription Factors
• Notch Pathway
• Pluripotent Stem Cell Transcription Factors
• Regulatory T Cells (Tregs)
• Smad Family
• Smad Transcription Factors
• TGF-beta Family
• TGF-beta Superfamily Modulators
• Transcription Factors in the Akt Pathway
• Transcription Regulators in VSMC