Human Phospho-p53 (S46) Antibody

Catalog #: MAB1489 Datasheet

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB1489
MAB1489-SP

Detection of Human Phospho-p53 (S46) by Western Blot.

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Human Phospho-p53 (S46) Antibody Summary

Species Reactivity

Human

Specificity

Detects human phospho-p53 (S46) in Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1022G

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Phosphopeptide containing the human p53 S46 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-p53 (S46) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 5 hours. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-p53 (S46) Monoclonal Antibody (Catalog # MAB1489) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for phospho-p53 (S46) at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p53

The p53 tumor suppressor protein signals apoptosis in cells that have incurred irreparable genotoxic damage. Phosphorylation of p53 at serine 46 is a critical event leading to the cellular decision to undergo apoptosis rather than cell cycle arrest. Serine 46 can be phosphorylated by either the p38 MAP kinase or the Homeodomain-Interacting Protein Kinase 2 (HIPK2).

Entrez Gene IDs

7157 (Human); 22059 (Mouse); 24842 (Rat)

Alternate Names

Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53