Human Phospho-p53 (S15) Antibody

Catalog #: AF1043
14 Citations 3 Reviews Datasheet

Catalog #	Availability	Size / Price	Qty
AF1043
AF1043-SP

Detection of Human Phospho-p53 (S15) by Western Blot.

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Citations (14)

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Reviews (3)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-p53 (S15) Antibody Summary

Species Reactivity

Human

Specificity

Detects human, monkey, and rat p53 when phosphorylated at S15 and mouse p53 when phosphorylated at S18.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human p53 S15 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Immunoprecipitation

1 µg/500 µg cell lysate

CEM human T-lymphoblastoid cell line exposed to UV-C and MCF‑7 human breast cancer cell line treated with camptothecin (CPT), see our available Western blot detection antibodies

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-p53 (S15) by Western Blot. Western blot shows lysates of CEM human T-lymphoblastoid cell line untreated or exposed to 100 J/m²UV-C for the indicated time. PVDF membrane was probed with 0.2 µg/mL Human Phospho-p53 (S15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1043) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S15) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. For additional reference, duplicate lysates were probed with 1:5000 dilution Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Phospho-p53 (S15) in HeLa Human Cell Line. p53 phosphorylated at S15 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with UV light (left panel) or untreated (right panel) using Rabbit Anti-Human Phospho-p53 (S15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1043) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Cells were co-stained using Rat Anti-Tubulin (Catalog # NB600-506, Novus Biologicals) and NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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Phospho-p53 (S15) in Human Breast Cancer Tissue. p53 phosphorylated at S15 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human Phospho-p53 (S15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1043) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

Immunohistochemistry

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Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p53

The p53 tumor suppressor protein is a multi-functional transcription factor that regulates proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Human p53 is a 393 amino acid (aa) nuclear/cytoplasmic protein that contains an N-terminal activation domain, a specific DNA binding domain, and a C-terminal domain that mediates tetramer formation and regulates DNA binding. P53 is phosphorylated on Ser15 in response to irradiation, and this phosphorylation increases recruitment of the coactivator CREB-binding protein/p300. The ATM or ATR kinases can phosphorylate p53 at serine 15 (S15), which leads to cell cycle arrest. Serine 15 phosphorylation leads to p53 stabilization and enhances transactivation of p53 target genes.

Entrez Gene IDs

7157 (Human); 22059 (Mouse); 24842 (Rat)

Alternate Names

Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human Phospho-p53 (S15) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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Prediction of breast cancer risk based on flow-variant analysis of circulating peripheral blood B cells.
Authors: Syeda MM, Upadhyay K, Loke J et al.
Genet Med
A p53/LINC00324 positive feedback loop suppresses tumor growth by counteracting SET-mediated transcriptional repression
Authors: Zhang, L;Zhang, J;Xuan, X;Wu, D;Yu, J;Wang, P;Yang, X;Zhang, J;Gan, W;He, M;Liu, XM;Zhou, J;Wang, D;Gu, W;Li, D;
Cell reports
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Transglutaminase 2 is associated with adverse colorectal cancer survival and represents a therapeutic target
Authors: Malkomes, P;Lunger, I;Oppermann, E;Lorenz, J;Faqar-Uz-Zaman, SF;Han, J;Bothur, S;Ziegler, P;Bankov, K;Wild, P;Bechstein, WO;Rieger, MA;
Cancer gene therapy
Species: Human
Sample Types: Tissue Microarray
Applications: IHC
Variation in Pentose Phosphate Pathway-associated Metabolism Dictates Cytotoxicity Outcomes Determined by Tetrazolium Reduction Assays
Authors: Coyle J, Johnson C, Jensen J et al.
Research Square
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Establishment and Characterization of Immortalized Miniature Pig Pancreatic Cell Lines Expressing Oncogenic K-RasG12D
Authors: HJ Yang, BS Song, BW Sim, Y Jung, U Chae, DG Lee, JJ Cha, SJ Baek, KS Lim, WS Choi, HY Lee, HC Son, SH Park, KJ Jeong, P Kang, SH Baek, BS Koo, HN Kim, YB Jin, YH Park, YK Choo, SU Kim
Int J Mol Sci, 2020-11-21;21(22):.
Species: Human
Sample Types: Cell Lysates
Impaired autophagy triggered by HDAC9 in mesenchymal stem cells accelerates bone mass loss
Authors: L Zhang, M Qi, J Chen, J Zhao, L Li, J Hu, Y Jin, W Liu
Stem Cell Res Ther, 2020-07-03;11(1):269.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Paneth Cell Multipotency Induced by Notch Activation following Injury
Authors: S Yu, K Tong, Y Zhao, I Balasubram, GS Yap, RP Ferraris, EM Bonder, MP Verzi, N Gao
Cell Stem Cell, 2018-06-07;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC
Hypoxia-Induced Cisplatin Resistance in Non-Small Cell Lung Cancer Cells Is Mediated by HIF-1Î± and Mutant p53 and Can Be Overcome by Induction of Oxidative Stress
Authors: C Deben, V Deschoolme, J De Waele, J Jacobs, J Van den Bo, A Wouters, M Peeters, C Rolfo, E Smits, F Lardon, P Pauwels
Cancers (Basel), 2018-04-21;10(4):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Skeletal muscle DNA damage precedes spinal motor neuron DNA damage in a mouse model of Spinal Muscular Atrophy (SMA).
Authors: Fayzullina, Saniya, Martin, Lee J
PLoS ONE, 2014-03-25;9(3):e93329.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Noninvasive assessment of regulable transferred-p53 gene expression and evaluation of therapeutic response with FDG-PET in tumor model.
Authors: Aung W, Hasegawa S, Koshikawa-Yano M
Gene Ther., 2010-05-06;17(9):1142-51.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

The p53 oncoprotein is a substrate for tissue transglutaminase kinase activity.
Authors: Mishra S, Murphy LJ
Biochem. Biophys. Res. Commun., 2005-11-28;339(2):726-30.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot

CCN2 Aggravates the Immediate Oxidative Stress-DNA Damage Response following Renal Ischemia-Reperfusion Injury
Authors: Valentijn FA, Knoppert SN, Pissas G et al.
Antioxidants (Basel, Switzerland)

PAI‐1 induction during kidney injury promotes fibrotic epithelial dysfunction via deregulation of klotho, p53, and TGF‐ beta 1‐receptor signaling
Authors: Cody C. Gifford, Fei Lian, Jiaqi Tang, Angelica Costello, Roel Goldschmeding, Rohan Samarakoon et al.
The FASEB Journal

Inhibition of a BTK-IDO-mTOR axis promotes differentiation of monocyte-lineage inflammatory dendritic cells and enhances anti-tumor T cell immunity
Authors: Madhav D. Sharma, Rafal Pacholczyk, Huidong Shi, Zuzana J. Berrong, Yousef Zakharia, Austin Greco et al.
Immunity