Human Phospho-p53 (S15) Antibody

Catalog #: MAB18391 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB18391
MAB18391-SP

Detection of Phospho-p53 in camptothecin-treated MCF‑7 Human Cell Line by Flow Cytometry.

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Human Phospho-p53 (S15) Antibody Summary

Species Reactivity

Human

Specificity

Detects human p53 phosphorylated at S15. No cross-reactivity with human p53 that is unphosphorylated at S15 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 261366

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Phosphopeptide containing the human p53 S15 site
Accession # P04637

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

Immersion fixed U2OS human osteosarcoma cell line

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Phospho-p53 antibody in camptothecin-treated MCF-7 Human Cell Line antibody by Flow Cytometry.

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Detection of Phospho-p53 in camptothecin-treated MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was unstimulated (light orange filled histogram) or treated with 1 µM camptothecin for 6 hours (dark orange filled histogram), then stained with Mouse Anti-Human Phospho-p53 (S15) Monoclonal Antibody (Catalog # MAB18391) or isotype control (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p53

The p53 tumor suppressor protein acts to enforce cell cycle checkpoints or signal apoptosis in cells that have incurred genotoxic damage. The ATM or ATR kinases can phosphorylate p53 at serine 15 to enforce cell cycle arrest. Serine 15 phosphorylation leads to p53 stabilization and enhances transactivation of p53 target genes.

Entrez Gene IDs

7157 (Human); 22059 (Mouse); 24842 (Rat)

Alternate Names

Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53