Human Phospho-p53 (S15) Alexa Fluor® 594-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: p53

The p53 tumor suppressor protein is a multi-functional transcription factor that regulates proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Human p53 is a 393 amino acid (aa) nuclear/cytoplasmic protein that contains an N-terminal activation domain, a specific DNA binding domain, and a C-terminal domain that mediates tetramer formation and regulates DNA binding. P53 is phosphorylated on Ser15 in response to irradiation, and this phosphorylation increases recruitment of the coactivator CREB-binding protein/p300. The ATM or ATR kinases can phosphorylate p53 at serine 15 (S15), which leads to cell cycle arrest. Serine 15 phosphorylation leads to p53 stabilization and enhances transactivation of p53 target genes.

Product Datasheets

Related Research Areas

• Apoptosis Transcription Factors and Regulators
• Cancer Biomarkers
• Cancer Stem Cell Transcription Factors
• Cell Cycle
• Checkpoint
• DNA Damage and Repair
• Epithelial Stem Cell Transcription Factors
• Hematopoietic Stem Cell Transcription Factors and Regulators
• Immunoglobulin-Like Domain Transcription Factors
• Mesenchymal Stem Cell Transcription Factors
• Neural Stem Cell Transcription Factors
• p53 Pathway
• Parkinson's Disease Related Molecules
• Pluripotent Stem Cell Transcription Factors
• TNF Superfamily Regulators
• Transcription Factors and Regulators
• Transcription Factors in the Akt Pathway