Human Phospho-p38 alpha (T180/Y182) Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB8691X Datasheet

Catalog #	Availability	Size / Price	Qty
FAB8691X-100UG

All p38 alpha Antibodies

Product Details

FAQs

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-p38 alpha (T180/Y182) Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human p38 alpha when phosphorylated at T180 and Y182.

Source

Monoclonal Mouse IgG2a Clone # 696602

Purification

Protein A or G purified

Immunogen

Phosphopeptide containing the human p38Î± T180/Y182 site
Accession # Q16539

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: p38 alpha

The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses. All four p38 family members, alpha, beta, gamma, and delta, are phosphorylated by MKK3 and/or MKK6 at dual Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr. Once activated, p38 phosphorylates a number of targets, including the nuclear transcription factors ATF2 and Max.

The most frequently analyzed family member, p38 alpha, also known as SAPK2a and MAPK14, was initially purified as a kinase critical to the signaling cascade linking IL-1 to MAPKAPK-2 and the small heat shock protein HSP27. Ubiquitously expressed, p38 alpha is dually phosphorylated by MKK3 and MKK6 at Thr180 and Tyr182. Once activated, p38 alpha phosphorylates a number of targets, including the cytoplasmic kinases MNK 4 and PRAK5 and the nuclear transcription factors ATF2 1 and STAT1. Several promising compounds that inhibit p38 alpha are being investigated as potential therapies for arthritic and inflammatory diseases.

Entrez Gene IDs

1432 (Human); 26416 (Mouse); 81649 (Rat)

Alternate Names

CSBP;CSBP1;CSBP2;CSPB1;EXIP;MAPK14;Mitogen-activated protein kinase;Mitogen-activated protein kinase 14;Mxi2;p38;p38ALPHA;PRKM14;PRKM15;RK;SAPK2A; MAPK14; p38 alpha; SAPK2a