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Human Phospho-p27/Kip1 (T198) Antibody

Catalog #: AF3994
19 Citations Datasheet
Catalog # Availability Size / Price Qty
AF3994
AF3994-SP
Detection of Human Phospho-p27/Kip1 (T198) by Western Blot.
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Citations (19)
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Human Phospho-p27/Kip1 (T198) Antibody Summary

Species Reactivity
Human
Specificity
Detects human p27/Kip1 when phosphorylated at T198. Does not recognize p27/Kip1 when unphosphorylated at T198.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human p27/Kip1 T198 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-p27/Kip1 (T198) antibody by Western Blot. View Larger

Detection of Human Phospho-p27/Kip1 (T198) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, non-transfected, transfected with plasmid expressing YFP-p27/Kip1 wild type (WT), or transfected with YFP-p27/Kip1 T198A mutant (T198A). PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-p27/Kip1 (T198) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3994) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p27/Kip1 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p27/Kip1

The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27/Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle. p27/Kip1 is phosphorylated at T198 in cells treated with the AMP-dependent protein kinase (AMPK) activator AICAR (5-amino-4‑imidazolecarboxamide riboside) or glucose withdrawal.

Long Name
p27 Cyclin Dependent Kinase 4 Inhibitor 1B
Entrez Gene IDs
1027 (Human); 12576 (Mouse); 83571 (Rat)
Alternate Names
CDKN1B; CDKN4; cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Kip1; KIP1P27KIP1; MEN1B; MEN4; p27; p27Kip1

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Citations for Human Phospho-p27/Kip1 (T198) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Role of dual specificity tyrosine-phosphorylation-regulated kinase 1B (Dyrk1B) in S-phase entry of HPV E7 expressing cells from quiescence
    Authors: Na Zhou, Shoudao Yuan, Rongchun Wang, Weifang Zhang, Jason J. Chen
    Oncotarget
  2. Hypoxia inducible prolyl hydroxylase PHD3 maintains carcinoma cell growth by decreasing the stability of p27.
    Authors: Hogel H, Miikkulainen P, Bino L, Jaakkola PM.
    Mol. Cancer.
  3. Mutant AKT1-E17K is oncogenic in lung epithelial cells
    Authors: Carmela De Marco, Donatella Malanga, Nicola Rinaldo, Fernanda De Vita, Marianna Scrima, Sara Lovisa et al.
    Oncotarget
  4. p27 controls Ragulator and mTOR activity in amino acid-deprived cells to regulate the autophagy-lysosomal pathway and coordinate cell cycle and cell growth
    Authors: A Nowosad, P Jeannot, C Callot, J Creff, RT Perchey, C Joffre, P Codogno, S Manenti, A Besson
    Nat. Cell Biol., 2020-08-17;22(9):1076-1090.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. CACYBP Enhances Cytoplasmic Retention of P27Kip1 to Promote Hepatocellular Carcinoma Progression in the Absence of RNF41 Mediated Degradation
    Authors: YF Lian, YL Huang, YJ Zhang, DM Chen, JL Wang, H Wei, YH Bi, ZW Jiang, P Li, MS Chen, YH Huang
    Theranostics, 2019-10-22;9(26):8392-8408.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Voluntary physical activity counteracts the proliferative tumor growth microenvironment created by adipose tissue via high-fat diet feeding in female rats
    Authors: CF Theriau, MK Connor
    Physiol Rep, 2017-07-01;5(13):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Cytoplasmic p27 promotes epithelial-mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation.
    Authors: Zhao D, Besser A, Wander S, Sun J, Zhou W, Wang B, Ince T, Durante M, Guo W, Mills G, Theodorescu D, Slingerland J
    Oncogene, 2015-02-16;34(43):5447-59.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  8. Antitumoral activity of lenalidomide in in vitro and in vivo models of mantle cell lymphoma involves the destabilization of cyclin D1/p27KIP1 complexes.
    Authors: Moros A, Bustany S, Cahu J, Saborit-Villarroya I, Martinez A, Colomer D, Sola B, Roue G
    Clin Cancer Res, 2013-10-31;20(2):393-403.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Immunoprecipitation, Western Blot
  9. TRIP6 regulates p27 KIP1 to promote tumorigenesis.
    Authors: Lin, Victor T, Lin, Vivian Y, Lai, Yun-Ju, Chen, Chen-Sha, Liu, Kang, Lin, Weei-Chi, Lin, Fang-Tsy
    Mol Cell Biol, 2013-01-22;33(7):1394-409.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. A novel p38-MAPK signaling axis modulates neutrophil biology in head and neck cancer.
    Authors: Dumitru CA, Fechner MK, Hoffmann TK
    J. Leukoc. Biol., 2012-01-18;91(4):591-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Cdc6 protein activates p27KIP1-bound Cdk2 protein only after the bound p27 protein undergoes C-terminal phosphorylation.
    Authors: Uranbileg B, Yamamoto H, Park JH
    J. Biol. Chem., 2012-01-05;287(9):6275-83.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Promotion of cytoplasmic mislocalization of p27 by Helicobacter pylori in gastric cancer.
    Authors: Wen S, So Y, Singh K
    Oncogene, 2011-08-15;31(14):1771-80.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Homeodomain-interacting Protein Kinase-2 Stabilizes p27kip1 by Its Phosphorylation at Serine 10 and Contributes to Cell Motility.
    Authors: Pierantoni GM, Esposito F, Tornincasa M, Rinaldo C, Viglietto G, Soddu S, Fusco A
    J. Biol. Chem., 2011-06-29;286(33):29005-13.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. The microbiome and butyrate regulate energy metabolism and autophagy in the mammalian colon.
    Authors: Donohoe DR, Garge N, Zhang X, Sun W, O'Connell TM, Bunger MK, Bultman SJ
    Cell Metab., 2011-05-04;13(5):517-26.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Ablation of Akt2 induces autophagy through cell cycle arrest, the downregulation of p70S6K, and the deregulation of mitochondria in MDA-MB231 cells.
    Authors: Santi SA, Lee H
    PLoS ONE, 2011-01-31;6(1):e14614.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Cyclin D1 regulates p27(Kip1) stability in B cells.
    Authors: Bustany S, Tchakarska G, Sola B
    Cell. Signal., 2010-09-15;23(1):171-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Cytoplasmic sequestration of p27 via AKT phosphorylation in renal cell carcinoma.
    Authors: Kim J, Jonasch E, Alexander A, Short JD, Cai S, Wen S, Tsavachidou D, Tamboli P, Czerniak BA, Do KA, Wu KJ, Marlow LA, Wood CG, Copland JA, Walker CL
    Clin. Cancer Res., 2009-01-01;15(1):81-90.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  18. PIM kinase inhibition presents a novel targeted therapy against triple-negative breast tumors with elevated MYC expression
    Authors: Dai Horiuchi, Roman Camarda, Alicia Y. Zhou, Christina Yau, Olga Momcilovic, Sanjeev Balakrishnan et al.
    Nature Medicine
  19. PCTAIRE1 phosphorylates p27 and regulates mitosis in cancer cells.
    Authors: Yanagi T, Krajewska M, Matsuzawa SI et al.
    Cancer Res

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