Human Phospho-IRS1 (Y612) Antibody

Catalog #: MAB7314 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB7314
MAB7314-SP

Detection of Human Phospho-IRS1 (Y612) by Western Blot.

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Human Phospho-IRS1 (Y612) Antibody Summary

Species Reactivity

Human

Specificity

Detects human IRS1 when phosphorylated at Y612 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 738662

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Phosphopeptide containing the human IRS1 Y612 site
Accession # P35568

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-IRS1 (Y612) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µg/mL Insulin for 5 minutes and 300 U/mL CIP for 1 hour. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Phospho-IRS1 (Y612) Monoclonal Antibody (Catalog # MAB7314) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-IRS1 (Y612) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRS1

Human IRS1 (insulin receptor substrate 1) is a 160-180 kDa substrate that transmits signals from the insulin and IGF-I receptor to downstream signaling modulators. The 1242 amino acid (aa) human IRS1 contains a PH domain (aa 12-115), a PTB domain (aa 160-263) and ten PEST sequences (aa 340-1225). IRS1 may be proteolytically cleaved at Arg656-Val657, generating a 90 kDa and 79 kDa fragment. Upon insulin/IGF-I receptor activation, IRS1 is tyrosine phosphorylated, allowing its association with PI-3 kinase and GRB2. Phosphorylation on Y612 and Y632 is important for IRS1 to fully activate PI3K and translocate GLUT4 in respose to insulin. The sequence surrounding pTyr612 is identical between human and mouse IRS1.

Long Name

Insulin Receptor Substrate 1

Entrez Gene IDs

3667 (Human); 16367 (Mouse); 25467 (Rat)

Alternate Names

HIRS-1; insulin receptor substrate 1; IRS1; IRS-1