Human Phospho-IRS1 (Y1179) Antibody

Catalog #: MAB7455 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB7455
MAB7455-SP

Detection of Human Phospho-IRS1 (Y1179) by Western Blot.

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Human Phospho-IRS1 (Y1179) Antibody Summary

Species Reactivity

Human

Specificity

Detects Human Phospho-IRS1 (Y1179) in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 744353

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Phosphopeptide containing the human IRS1 Y1179 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-IRS1 (Y1179) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µg/mL Insulin for 5 minutes and 300 U/mL CIP for 1 hour. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-IRS1 (Y1179) Monoclonal Antibody (Catalog # MAB7455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-IRS1 (Y1179) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Phospho-IRS1 (Y1179) in MCF‑7 Human Cell Line. IRS1 phosphorylated at Y1179 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Phospho-IRS1 (Y1179) Monoclonal Antibody (Catalog # MAB7455) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRS1

Human IRS1 (insulin receptor substrate 1) is a 160-180 kDa substrate intermediate between the insulin and IGF-I receptor, and downstream signaling modulators. Upon insulin/IGF-I receptor activation, IRS1 is tyrosine phosphorylated, allowing its association with PI-3 kinase and GRB2. Human IRS1 is 1242 amino acids (aa) in length. It contains a PH (pleckstrin homology) domain (aa 12-115), followed by a PTB (phosphotyrosine-binding) domain (aa 160-263) and ten PEST (Pro/Glu/Ser/Thr) regions (aa 340-1225). IRS1 may be proteolytically cleaved at Arg656-Val657, generating a 90 kDa and 79 kDa fragment. Insulin receptor-mediated phosphorylation of human IRS1 on Y1179, or its murine equivalent, Y1172, mediates IRS-1 interaction with SH2 domains such as that of the Src family kinase, Fyn, or the tyrosine phosphatase, SHP-2.

Long Name

Insulin Receptor Substrate 1

Entrez Gene IDs

3667 (Human); 16367 (Mouse); 25467 (Rat)

Alternate Names

HIRS-1; insulin receptor substrate 1; IRS1; IRS-1