Human Phospho-IRS1 (S616) Antibody

Catalog #: MAB39783 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB39783
MAB39783-SP

Detection of Human Phospho-IRS1 (S616) by Western Blot.

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Human Phospho-IRS1 (S616) Antibody Summary

Species Reactivity

Human

Specificity

Detects human IRS1 when phosphorylated at S616.

Source

Monoclonal Mouse IgG_2A Clone # 738710

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Phosphopeptide containing the human IRS1 S616 site
Accession # P35568

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-IRS1 (S616) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µg/mL Insulin for 5 minutes and 300 U/mL CIP for 1 hour. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-IRS1 (S616) Monoclonal Antibody (Catalog # MAB39783) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-IRS1 (S616) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRS1

Human IRS1 (insulin receptor substrate 1) is a 150-180 kDa substrate that transmits signals from the insulin and IGF-I receptor to downstream signaling modulators. The 1242 amino acid (aa) human IRS1 contains a PH domain (aa 12-115), a PTB domain (aa 160-263) and ten PEST sequences (aa 340-1225). IRS1 may be proteolytically cleaved at Arg656-Val657, generating a 90 kDa and 79 kDa fragment. Upon insulin/IGF-I receptor activation, IRS1 is tyrosine phosphorylated, allowing its association with PI-3 kinase and GRB2. When PI-3 kinase and ERK1/2 are activated, insulin or angiotensin II can also stimulate phosphorylation on Ser616. The aa sequence surrounding pSer616 is identical between human, mouse and rat IRS-1.

Long Name

Insulin Receptor Substrate 1

Entrez Gene IDs

3667 (Human); 16367 (Mouse); 25467 (Rat)

Alternate Names

HIRS-1; insulin receptor substrate 1; IRS1; IRS-1