Human Phospho-ErbB3/Her3 (Y1262) Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human Phospho-ErbB3/Her3 (Y1262) by Western Blot. Western blot shows lysates of MDA-MB-453 human breast cancer cell line untreated (-) or treated (+) with 300 ng/mL Recombinant Human NRG1-beta /HRG1-beta (Catalog # 396-HB) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Human Phospho-ErbB3/Her3 (Y1262) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5817), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-ErbB3/Her3 (Y1262) at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ErbB3/Her3
ErbB3 (also known as HER3) is a type I transmembrane growth factor receptor and member of the human epidermal growth factor receptor (HER) family. ErbB3 is unique among HER family members in that it contains a defective kinase domain, and is therefore active only as a partner in a heterodimer complex with other ErbBs. Despite ErbB3's lack of intrinsic kinase activity, these heterodimers can be quite potent: the ErbB2/ErbB3 complex is considered the most active ErbB signaling dimer for the activation of downstream pathways. In addition, monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG), while ErbB2/ErbB3 heterodimers form a high affinity receptor complex. Phosphorylation of ErbB3 at Y1262 following ligand binding generates a binding site for the Grb2 adaptor protein.
Product Datasheets
Citations for Human Phospho-ErbB3/Her3 (Y1262) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
4
Citations: Showing 1 - 4
Filter your results:
Filter by:
-
Leucine Zipper Downregulated in Cancer-1 Interacts with Clathrin Adaptors to Control Epidermal Growth Factor Receptor (EGFR) Internalization and Gefitinib Response in EGFR-Mutated Non-Small Cell Lung Cancer
Authors: Huang, HN;Hung, PF;Chen, YP;Lee, CH;
International journal of molecular sciences
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
p52Shc regulates the sustainability of ERK activation in a RAF-independent manner
Authors: Ryo Yoshizawa, Nobuhisa Umeki, Akihiro Yamamoto, Mariko Okada, Masayuki Murata, Yasushi Sako
Molecular Biology of the Cell
-
Biphasic spatiotemporal regulation of GRB2 dynamics by p52SHC for transient RAS activation
Authors: Ryo Yoshizawa, Nobuhisa Umeki, Akihiro Yamamoto, Masayuki Murata, Yasushi Sako
Biophysics and Physicobiology
-
Quantitative Phosphoproteomics Analysis of ERBB3/ERBB4 Signaling
Authors: Sebastian K. Wandinger, Idoya Lahortiga, Kris Jacobs, Martin Klammer, Nicole Jordan, Sarah Elschenbroich et al.
PLOS ONE
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Phospho-ErbB3/Her3 (Y1262) Antibody
There are currently no reviews for this product. Be the first to review Human Phospho-ErbB3/Her3 (Y1262) Antibody and earn rewards!
Have you used Human Phospho-ErbB3/Her3 (Y1262) Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
