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Human Phospho-EGFR (Y1068) Antibody

Catalog #: MAB3570
9 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
MAB3570
MAB3570-SP
Detection of Human Phospho-EGFR (Y1068) by Western Blot.
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Product Details
Citations (9)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-EGFR (Y1068) Antibody Summary

Species Reactivity
Human
Specificity
Detects human EGFR phosphorylated at Y1068.
Source
Monoclonal Mouse IgG2A Clone # 338324
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing human EGFR Y1068 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-EGFR (Y1068) antibody by Western Blot. View Larger

Detection of Human Phospho-EGFR (Y1068) by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Human Phospho-EGFR (Y1068) Monoclonal Antibody (Catalog # MAB3570), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-EGFR (Y1068) at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry Detection of Phospho-EGFR antibody in EGF-treated A431 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Phospho-EGFR in EGF-treated A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was untreated (open histogram) or treated for 5 minutes with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG, filled histogram) then stained with Human Phospho--EGFR (Y1068) Monoclonal Antibody (Catalog # MAB3570), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). Mouse IgG2A(Catalog # MAB003, data not shown) was used as an isotype control. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

Epidermal growth factor receptor (EGFR, also known as ErbB1 and HER1) is the founding member of the ErbB family of receptor tyrosine kinases. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Phosphorylation at Tyr 1068 allows binding of the SH2 domain of the cytosolic adaptor Grb2. This binding results in Ras activation.

Long Name
Epidermal Growth Factor Receptor
Entrez Gene IDs
1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)
Alternate Names
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

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Citations for Human Phospho-EGFR (Y1068) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Chemical probe mediated visualization of protein S-palmitoylation in patient tissue samples
    Authors: Nancy Schek, Jia-Ying Lee, George M. Burslem, Eric Witze
    Frontiers in Physiology
  2. BEBT-109, a pan-mutant-selective EGFR inhibitor with potent antitumor activity in EGFR-mutant non-small cell lung cancer
    Authors: F Fan, M Zhou, X Ye, Z Mo, Y Ma, L Luo, X Liang, H Liu, Y Weng, M Lin, X Liu, X Cai, C Qian
    Translational Oncology, 2020-12-13;14(2):100961.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Counting growth factors in single cells with infrared quantum dots to measure discrete stimulation distributions
    Authors: P Le, SJ Lim, BC Baculis, HJ Chung, KA Kilian, AM Smith
    Nat Commun, 2019-02-22;10(1):909.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Dopamine and its receptors play a role in the modulation of CCR5 expression in innate immune cells following exposure to Methamphetamine: Implications to HIV infection
    Authors: L Basova, JA Najera, N Bortell, D Wang, R Moya, A Lindsey, S Semenova, RJ Ellis, MCG Marcondes
    PLoS ONE, 2018-06-26;13(6):e0199861.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. GBM heterogeneity as a function of variable epidermal growth factor receptor variant III activity
    Authors: Olle R. Lindberg, Andrew McKinney, Jane R. Engler, Gayane Koshkakaryan, Henry Gong, Aaron E. Robinson et al.
    Oncotarget
  6. Activation of EGFR by small compounds through coupling the generation of hydrogen peroxide to stable dimerization of Cu/Zn SOD1.
    Authors: Sakanyan V, Hulin P, Alves de Sousa R, Silva V, Hambardzumyan A, Nedellec S, Tomasoni C, Loge C, Pineau C, Roussakis C, Fleury F, Artaud I
    Sci Rep, 2016-02-17;6(0):21088.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. AZD9291, an Irreversible EGFR TKI, Overcomes T790M-Mediated Resistance to EGFR Inhibitors in Lung Cancer
    Authors: Darren A. E. Cross, Susan E. Ashton, Serban Ghiorghiu, Cath Eberlein, Caroline A. Nebhan, Paula J. Spitzler et al.
    Cancer Discovery
  8. S100A2 promoter-driven conditionally replicative adenovirus targets non-small-cell lung carcinoma.
    Mol Cell, 2011-10-27;19(10):967-77.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Mechanism of intrinsic resistance of lung squamous cell carcinoma to epithelial growth factor receptor‑tyrosine kinase inhibitors revealed by high‑throughput RNA interference screening
    Authors: Lixia Ju, Zhiyi Dong, Juan Yang, Minghua Li
    Oncology Letters

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Human Phospho-EGFR (Y1068) Antibody
By Anonymous on 12/07/2020
Application: MiAr Sample Tested: EDTA Plasma Species: Human
Microarray Human Phospho-EGFR (Y1068) Antibody MAB3570