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Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Antibody

Catalog #: MAB25382
13 Citations Datasheet
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB25382
MAB25382-SP
Detection of Human Phospho-DDR2 (Y740) by Western Blot.
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Product Details
Citations (13)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Antibody Summary

Species Reactivity
Human
Specificity
Detects human DDR1 and DDR2 when phosphorylated at Y796 and Y740.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1119D
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phosphopeptide containing the human DDR2 Y740 site
Accession # Q16832
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-DDR2 (Y740) antibody by Western Blot. View Larger

Detection of Human Phospho-DDR2 (Y740) by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human DDR2 untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) for 10 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Monoclonal Antibody (Catalog # MAB25382) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-DDR2 (Y740) at approximately 120-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Phospho-DDR2 (Y740) antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Phospho-DDR2 (Y740) by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with human DDR2 untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-DDR2 (Y740) at approximately 130 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Monoclonal Antibody (Catalog # MAB25382). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DDR1/DDR2

DDR2, also known as TYR010 and TKT, is a widely expressed 130 kDa type I transmembrane glycoprotein belonging to the discoidin-like domain containing subfamily of receptor tyrosine kinases (1). Mature human DDR2 consists of a 378 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 22 aa transmembrane segment, and a 434 aa cytoplasmic domain that includes the kinase domain (2). Within the ECD, human DDR2 shares 53% aa sequence identity with DDR1 and 97% aa sequence identity with mouse DDR2. The discoidin-like domain mediates DDR2 interactions with collagens I, III, and X (3-5). Collagens II and V are less efficacious ligands (3). DDR2 selectively recognizes the triple helical structure of collagen compared to monomeric or denatured collagen (3, 5, 6). Within collagen II, the D2 period is required for DDR2 binding, and the D1 period is additionally required to trigger DDR2 autophosphorylation (6). The ECD of DDR2 exists as a non-covalent dimer in solution, and dimerization of the receptor greatly enhances collagen binding (4, 7). DDR2 interaction with collagen I inhibits collagen fibrillogenesis and alters collagen fiber morphology (7). Ligand binding induces DDR2 autophosphorylation in the cytoplasmic domain (3, 5, 8), which promotes associations with Shc and Src (9). In addition to the above mechanism, DDR2 exhibits a distinct interaction with collagen X. A region other than the discoidin-like domain of DDR2 recognizes the non-helical NC1 domain of collagen X, and this interaction does not lead to receptor autophosphorylation (5). Activation of DDR2 by collagen induces upregulation of MMP-1, -2, and -13 as well as DDR2 itself (3, 8, 10). DDR2 is implicated in collagenous matrix destruction and cell invasiveness (8, 10). DDR2 is also upregulated in several pathological conditions, including hepatic fibrosis following injury, rheumatoid and osteoarthritis, and smooth muscle cell hyperplasia (8, 10-12).

References
  1. Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
  2. Karn, T. et al. (1993) Oncogene 8:3433.
  3. Vogel, W. et al. (1997) Mol. Cell 1:13.
  4. Leitinger, B. (2003) J. Biol. Chem. 278:16761.
  5. Leitinger, B. and A.P.L Kwan (2006) Matrix Biol. 25:355.
  6. Leitinger, B. et al. (2004) J. Mol. Biol. 344:993.
  7. Mihai, C. et al. (2006) J. Mol. Biol. 361:864.
  8. Olaso, E. et al. (2001) J. Clin. Invest. 108:1369.
  9. Ikeda, K. et al. (2002) J. Biol. Chem. 277:19206.
  10. Xu, L. et al. (2005) J. Biol. Chem. 280:548.
  11. Wang, J. et al. (2002) J. Autoimmun. 19:161.
  12. Ferri, N. et al. (2004) Am. J. Pathol. 164:1575.
Long Name
Discoidin Domain Receptor 1/Discoidin Domain Receptor 2
Alternate Names
6030432F18;AI323681;CAK;CD167;CD167a;DDR;DDR1;Drd1;EDDR1;HGK2;MCK10;Mpk6;NEP;NTRK4;PTK3;PTK3A;PTK3D;RTK6;TRKE; DDR1/DDR2

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Citations for Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. DDR2 facilitates hepatocellular carcinoma invasion and metastasis via activating ERK signaling and stabilizing SNAIL1.
    Authors: Xie B, Lin W, Ye J, Wang X, Zhang B, Xiong S, Li H, Tan G
    J Exp Clin Cancer Res, 2015-09-11;34(0):101.
  2. Control of craniofacial development by the collagen receptor, discoidin domain receptor 2
    Authors: Mohamed FF, Ge C, Hallett SA et al.
    eLife
  3. Discoidin domain receptor 2 regulates aberrant mesenchymal lineage cell fate and matrix organization
    Authors: CA Pagani, AC Bancroft, RJ Tower, N Livingston, Y Sun, JY Hong, RN Kent, AL Strong, JH Nunez, JMR Medrano, N Patel, BA Nanes, KM Dean, Z Li, C Ge, BM Baker, AW James, SJ Weiss, RT Franceschi, B Levi
    Science Advances, 2022-12-21;8(51):eabq6152.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC/IF
  4. Discoidin Domain Receptor 2 orchestrates melanoma resistance combining phenotype switching and proliferation
    Authors: M Sala, N Allain, M Moreau, A Jabouille, E Henriet, A Abou-Hammo, A Uguen, S Di-Tommaso, C Dourthe, AA Raymond, JW Dupuy, E Gerard, N Dugot-Sena, B Rousseau, JP Merlio, A Pham-Ledar, B Vergier, S Tartare-De, V Moreau, F Saltel
    Oncogene, 2022-03-23;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Tyrosine kinase independent actions of DDR2 in tumor cells and CAFs influence tumor invasion, migration, and metastasis
    Authors: CE Barcus, PY Hwang, V Morikis, A Brenot, P Pence, M Clarke, GD Longmore
    Journal of Cell Science, 2021-10-13;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. DDR2 upregulation confers ferroptosis susceptibility of recurrent breast tumors through the Hippo pathway
    Authors: CC Lin, WH Yang, YT Lin, X Tang, PH Chen, CC Ding, DC Qu, JV Alvarez, JT Chi
    Oncogene, 2021-02-18;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Knockdown of AKT3 Activates HER2 and DDR Kinases in Bone-Seeking Breast Cancer Cells, Promotes Metastasis In Vivo and Attenuates the TGF&beta/CTGF Axis
    Authors: N Hinz, A Baranowsky, M Horn, M Kriegs, F Sibbertsen, DJ Smit, P Clezardin, T Lange, T Schinke, M Jücker
    Cells, 2021-02-18;10(2):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Two-step release of kinase autoinhibition in discoidin domain receptor 1
    Authors: D Sammon, E Hohenester, B Leitinger
    Proc. Natl. Acad. Sci. U.S.A., 2020-08-24;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Protein
    Applications: Western Blot
  9. Chain alignment of collagen I deciphered using computationally designed heterotrimers
    Authors: AA Jalan, D Sammon, JD Hartgerink, P Brear, K Stott, SW Hamaia, EJ Hunter, DR Walker, B Leitinger, RW Farndale
    Nat. Chem. Biol., 2020-01-06;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. DDR1 autophosphorylation is a result of aggregation into dense clusters
    Authors: DS Corcoran, V Juskaite, Y Xu, F Görlitz, Y Alexandrov, C Dunsby, PMW French, B Leitinger
    Sci Rep, 2019-11-19;9(1):17104.
    Species: African Green Monkey
    Sample Types: Whole Cells
    Applications: ICC
  11. Parity predisposes breasts to the oncogenic action of PAPP-A and activation of the collagen receptor DDR2
    Authors: E Slocum, A Craig, A Villanueva, D Germain
    Breast Cancer Res., 2019-05-02;21(1):56.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Clustering, Spatial Distribution and Phosphorylation of Discoidin Domain Receptors 1 and 2 in Response to Soluble Collagen Type I
    Authors: David A. Yeung, Nirvan Shanker, Anjum Sohail, Brent A. Weiss, Carolyn Wang, Jack Wellmerling et al.
    Journal of Molecular Biology
  13. Mesenchymal Stem Cell-Induced DDR2 Mediates Stromal-Breast Cancer Interactions and Metastasis Growth
    Authors: ME Gonzalez, EE Martin, T Anwar, C Arellano-G, N Medhora, A Lama, YC Chen, KS Tanager, E Yoon, KM Kidwell, C Ge, RT Franceschi, CG Kleer
    Cell Rep, 2017-01-31;18(5):1215-1228.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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