Human Phospho-Chk1 (S345) Alexa Fluor® 700-conjugated Antibody

Catalog #: AF2475N Datasheet

Catalog #	Availability	Size / Price	Qty
AF2475N-100UG

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Human Phospho-Chk1 (S345) Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human, mouse, and rat Chk1 when phosphorylated at S345. This antibody does not recognize Chk1 when unphosphorylated at S345.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human Chk1 S345 site

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Chk1

The Chk1 checkpoint kinase is an integral member of a signaling cascade that controls cell cycle progression. In response to genotoxic or replicative stress, Chk1 is phosphorylated by the ATM or ATM-related kinase (ATR) at S345. In turn, Chk1 phosphorylates downstream effectors, such as p53 or the Cdc25 phosphatases to halt cell cycle progression and allow time for repair of incurred damage.

Long Name

Checkpoint Kinase 1

Entrez Gene IDs

1111 (Human); 12649 (Mouse); 140583 (Rat)

Alternate Names

Checkpoint, S. pombe, homolog of, 1; CHEK1; CHK1 (checkpoint, S.pombe) homolog; CHK1 checkpoint homolog (S. pombe); Chk1; CHK1serine/threonine-protein kinase Chk1; EC 2.7.11; EC 2.7.11.1

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