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Human PDGF R beta PE-conjugated Antibody

Catalog #: FAB1263P
13 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
FAB1263P
Detection of PDGF R beta in MG‑63 Human Cell Line by Flow Cytometry.
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Citations (13)
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Reviews (1)
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Human PDGF R beta PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDGF R beta in Western blots. Specificity has been confirmed in binding studies using several different cell lines (1, 2) and by its ability to immunoprecipitate PDGF R beta -subunit complexed with 125I‑PDGF-BB (3). It does not recognize the PDGF R alpha ‑subunit.
Detects the PDGF R beta -subunit of human and primate species (monkey and baboon) but not the rat or mouse receptors. Its ability to bind to receptors from other species has not been tested.
Source
Monoclonal Mouse IgG1 Clone # PR7212
Purification
Protein A or G purified from ascites
Immunogen
Human skin fibroblast membrane extracts
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PDGF R beta antibody in MG-63 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of PDGF R beta in MG‑63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human PDGF R beta PE-conjugated Monoclonal Antibody (Catalog # FAB1263P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PDGF R beta

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta  are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt-3 Ligand. All class III RTKs are characterized by the presence of five immunoglobulin‑like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo- and hetero‑dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is a potent PDGF antagonist (4).

References
  1. Hart et al. (1987) J. Biol. Chem. 262:10780.
  2. Gronwald et al. (1988) Proc. Natl. Acad. Sci. 85:3435.
  3. Seifert et al. (1989) J. Biol. Chem. 264:8771.
  4. Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.
Long Name
Platelet-derived Growth Factor Receptor beta
Entrez Gene IDs
5159 (Human); 18596 (Mouse); 24629 (Rat)
Alternate Names
beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide

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Citations for Human PDGF R beta PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Transient and Prolonged Activation of Wnt Signaling Contribute Oppositely to the Pathogenesis of Asherman's Syndrome
    Authors: X Xue, X Li, J Yao, X Zhang, X Ren, S Xu
    International Journal of Molecular Sciences, 2022-08-08;23(15):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  2. Hypoxia Regulates the Self-Renewal of Endometrial Mesenchymal Stromal/Stem-like Cells via Notch Signaling
    Authors: S Zhang, RWS Chan, EHY Ng, WSB Yeung
    International Journal of Molecular Sciences, 2022-04-21;23(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Magnetic Bead Selection
  3. Spatial and temporal characterization of endometrial mesenchymal stem-like cells activity during the menstrual cycle
    Authors: William S B Yeung
    Exp. Cell Res., 2016-11-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. A Chemically Defined Medium-Based Strategy to Efficiently Generate Clinically Relevant Cord Blood Mesenchymal Stromal Colonies
    Authors: Mario Barilani, Cristiana Lavazza, Valentina Boldrin, Enrico Ragni, Valentina Parazzi, Mariacristina Crosti et al.
    Cell Transplantation
  5. Inhibition of Transforming Growth Factor-beta Receptor signaling promotes culture expansion of undifferentiated human Endometrial Mesenchymal Stem/stromal Cells
    Authors: Shanti Gurung, Jerome A. Werkmeister, Caroline E. Gargett
    Scientific Reports
  6. PDGF-induced proliferation in human arterial and venous smooth muscle cells: Molecular basis for differential effects of PDGF isoforms
    Authors: Li Li, Donald K. Blumenthal, Christi M. Terry, Yuxia He, Mary L. Carlson, Alfred K. Cheung
    Journal of Cellular Biochemistry
  7. Dissection of the Cord Blood Stromal Component Reveals Predictive Parameters for Culture Outcome
    Authors: Mario Barilani, Cristiana Lavazza, Mariele Viganò, Tiziana Montemurro, Valentina Boldrin, Valentina Parazzi et al.
    Stem Cells and Development
  8. Hypoxia-controlled EphA3 marks a human endometrium-derived multipotent mesenchymal stromal cell that supports vascular growth.
    Authors: To C, Farnsworth R, Vail M, Chheang C, Gargett C, Murone C, Llerena C, Major A, Scott A, Janes P, Lackmann M
    PLoS ONE, 2014-11-24;9(11):e112106.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Antiangiogenic cancer drug sunitinib exhibits unexpected proangiogenic effects on endothelial cells.
    Authors: Norton, Kerri-An, Han, Zheyi, Popel, Aleksand, Pandey, Niranjan
    Onco Targets Ther, 2014-09-09;7(0):1571-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Lipopolysaccharide activates Toll-like receptor 4 (TLR4)-mediated NF-kappaB signaling pathway and proinflammatory response in human pericytes.
    Authors: Guijarro-Munoz I, Compte M, Alvarez-Cienfuegos A, Alvarez-Vallina L, Sanz L
    J Biol Chem, 2013-12-04;289(4):2457-68.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Bone marrow fibroblasts parallel multiple myeloma progression in patients and mice: in vitro and in vivo studies.
    Authors: Frassanito M, Rao L, Moschetta M, Ria R, di Marzo L, De Luisi A, Racanelli V, Catacchio I, Berardi S, Basile A, Menu E, Ruggieri S, Nico B, Ribatti D, Fumarulo R, Dammacco F, Vanderkerken K, Vacca A
    2013-09-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium.
    Authors: Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G
    PLoS ONE, 2012-10-09;7(10):e47187.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis.
    Authors: Guijarro-Munoz I, Cuesta AM, Alvarez-Cienfuegos A, Geng JG, Alvarez-Vallina L, Sanz L
    Exp. Cell Res., 2011-12-14;318(4):371-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human PDGF R beta PE-conjugated Antibody
By Alina Filatova on 10/26/2017
Application: Flow Sample Tested: G55TL Glioblastoma cell line Species: Human
Flow Cytometry Human PDGF R beta PE-conjugated Antibody FAB1263P