Human PDGF R alpha (Research Grade Olaratumab Biosimilar) Antibody

Catalog #: MAB10352 Datasheet
Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
MAB10352-100
MAB10352-SP
Detection of PDGF R alpha in U-118-MG human glioblastoma cell line by Flow Cytometry.
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Human PDGF R alpha (Research Grade Olaratumab Biosimilar) Antibody Summary

Species Reactivity
Human
Specificity
Detects Human PDGF R alpha in direct ELISAs.This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Olaratumab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu136
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human PDGF R alpha
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PDGF R alpha antibody in U-118-MG human glioblastoma cell line antibody by Flow Cytometry. View Larger

Detection of PDGF R alpha in U-118-MG human glioblastoma cell line by Flow Cytometry. U-118-MG human glioblastoma cell line was stained with Human Anti-Human PDGF R alpha (Research Grade Olaratumab Biosimilar) Monoclonal Antibody (Catalog # MAB10352, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF R alpha

Olaratumab is a biosimilar directed against human PDGF R alpha.

Long Name
Platelet-derived Growth Factor Receptor alpha
Entrez Gene IDs
5156 (Human); 18595 (Mouse)
Alternate Names
alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA

Product Datasheets

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FAQs

  1. Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

    • The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.

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