Human PDGF R alpha PE-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of PDGF R alpha in U‑118‑MG Human Cell Line by Flow Cytometry. U-118-MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human PDGF Ra PE-conjugated Monoclonal Antibody (Catalog # FAB1264P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PDGF R alpha
PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF‑BB and AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGF-R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).
- Heldin, C.H. and L. Claesson-Welsh (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A. (ed) Oxford University Press, New York, NY p. 202.
Product Datasheets
Citations for Human PDGF R alpha PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Modeling human multi-lineage heart field development with pluripotent stem cells
Authors: D Yang, J Gomez-Garc, S Funakoshi, T Tran, I Fernandes, GD Bader, MA Laflamme, GM Keller
Cell Stem Cell, 2022-09-01;29(9):1382-1401.e8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human Pluripotent Stem Cell-Derived Atrial and Ventricular Cardiomyocytes Develop from Distinct Mesoderm Populations
Authors: JH Lee, SI Protze, Z Laksman, PH Backx, GM Keller
Cell Stem Cell, 2017-08-03;21(2):179-194.e4.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A derivative of platelet-derived growth factor receptor alpha binds to the trimer of human cytomegalovirus and inhibits entry into fibroblasts and endothelial cells
Authors: C Stegmann, D Hochdorfer, D Lieber, N Subramania, D Stöhr, K Laib Sampa, C Sinzger
PLoS Pathog., 2017-04-12;13(4):e1006273.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
PDGF-induced proliferation in human arterial and venous smooth muscle cells: Molecular basis for differential effects of PDGF isoforms
Authors: Li Li, Donald K. Blumenthal, Christi M. Terry, Yuxia He, Mary L. Carlson, Alfred K. Cheung
Journal of Cellular Biochemistry
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Chemotherapy activates cancer-associated fibroblasts to maintain colorectal cancer-initiating cells by IL-17A.
Authors: Lotti, Fiorenza, Jarrar, Awad M, Pai, Rish K, Hitomi, Masahiro, Lathia, Justin, Mace, Adam, Gantt, Gerald A, Sukhdeo, Kumar, DeVecchio, Jennifer, Vasanji, Amit, Leahy, Patrick, Hjelmeland, Anita B, Kalady, Matthew, Rich, Jeremy N
J Exp Med, 2013-12-09;210(13):2851-72.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Direct contact with endoderm-like cells efficiently induces cardiac progenitors from mouse and human pluripotent stem cells.
Authors: Uosaki, Hideki, Andersen, Peter, Shenje, Lincoln, Fernandez, Laviel, Christiansen, Sofie Li, Kwon, Chulan
PLoS ONE, 2012-10-02;7(10):e46413.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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