Home / PDGF R alpha / Human PDGF R alpha Antibody

Human PDGF R alpha Antibody

Catalog #: AF-307-NA
57 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
AF-307-NA
AF-307-SP
Cell Proliferation Induced by PDGF‑AA and Neutralization by Human PDGF R alpha Antibody.
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Citations (57)
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Human PDGF R alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse PDGF R alpha, recombinant human (rh) PDGF R beta, rhFGF R2, and rhFGF R3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PDGF R alpha
Gln24-Glu524
Accession # P16234
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human PDGF R alpha (Catalog # 322-PR)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize PDGF‑AA-induced proliferation in the WS-1 human fetal skin fibroblast cell line. The Neutralization Dose (ND50) is typically 1-6 µg/mL in the presence of 10 ng/mL Recombinant Human PDGF‑AA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by PDGF‑AA and Neutralization by Human PDGF R alpha Antibody. View Larger

Cell Proliferation Induced by PDGF‑AA and Neutralization by Human PDGF R alpha Antibody. Recombinant Human PDGF-AA (Catalog # 221-AA) stimulates proliferation in the WS-1 human fetal skin fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-AA (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-307-NA). The ND50 is typically 1-6 µg/mL.

Immunohistochemistry PDGF Ra antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

PDGF R alpha in Human Breast Cancer Tissue. PDGF Ra was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-307-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry PDGF Ra antibody in Human Ovary by Immunohistochemistry (IHC-P). View Larger

PDGF R alpha in Human Ovary. PDGF Ra was detected in immersion fixed paraffin-embedded sections of human ovarian array using Goat Anti-Human PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-307-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry PDGF Ra antibody in Human Osteosarcoma by Immunohistochemistry (IHC-P). View Larger

PDGF R alpha in Human Osteosarcoma. PDGF Ra was detected in immersion fixed paraffin-embedded sections of human osteosarcoma using Goat Anti-Human PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-307-NA) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry/ Immunofluorescence Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence Representative light and fluorescence microscopy photomicrographs of normal human corneal sections. (A–C) CD34 immunoperoxidase‐based immunohistochemistry with haematoxylin counterstain. (A) CD34‐positive stromal cells are orderly arranged and parallel to the corneal surface. (B and C) At higher magnification, the CD34‐positive stromal cells appear as spindle‐shaped cells with a small oval body and typically two long and thin moniliform cell processes characterized by the alternation of slender segments (arrows) and knobs/dilations (arrowheads) along their length. (D–I) Double immunofluorescence labelling for CD34 (red) and PDGFR alpha (green) with DAPI (blue) counterstain for nuclei. Colocalization of CD34 and PDGFR alpha in stromal cells gives rise to yellow staining either in the anterior corneal stroma (D–F) or in the deeper corneal stromal layer (G–I). CD34+/PDGFR alpha + stromal cells display cell morphologies very evocative for telocytes: a small cell body with very long prolongations (telopodes) characterized by a moniliform silhouette with the alternation of podoms (arrowheads) and podomers (arrows). (J–L) Double immunofluorescence labelling for CD34 (red) and c‐kit (green) with DAPI (blue) counterstain for nuclei. Either in the subepithelial corneal stroma or in the deeper stromal layer, numerous CD34‐positive stromal cells coexpress c‐kit. Inset: Higher magnification view of CD34+/c‐kit+ corneal stromal cells. Scale bar: 50 μm (A, D–F and J–L), 25 μm (B, C and G–I). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28714595), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence Representative light and fluorescence microscopy photomicrographs of normal and keratoconic human corneal sections. (A and B) CD34 immunoperoxidase‐based immunohistochemistry with haematoxylin counterstain. (A) In control normal corneas, CD34‐positive stromal cells displaying morphological features of telocytes are orderly arranged and parallel to the corneal surface. (B) In keratoconus, a patchy loss of CD34‐positive stromal cells is mostly evident in the anterior corneal stroma. Insets: Higher magnification views of CD34‐positive corneal stromal cells. (C) Results of quantitative analysis of CD34‐positive telocyte counts per high‐power field in the corneal stroma of healthy controls (n = 6) and patients with keratoconus (n = 6). Data are mean ± S.D. *P < 0.001 versus control. (D–I) Double immunofluorescence labelling for CD34 (red) and PDGFR alpha (green) with DAPI (blue) counterstain for nuclei. (D–F) In control normal corneas, CD34+/PDGFR alpha + stromal cells (telocytes) are orderly distributed throughout the stromal compartment. (G–I) In keratoconic corneas, a patchy loss of CD34+/PDGFR alpha + stromal cells (telocytes) is mainly evident in the subepithelial stroma. (J and K) Double immunofluorescence labelling for CD34 (red) and c‐kit (green) with DAPI (blue) counterstain for nuclei. (J) In control normal corneas, numerous CD34+/c‐kit+ stromal cells (telocytes) are present throughout the stromal layer. (K) In keratoconic corneas, the CD34+/c‐kit+ stromal cell subpopulation is almost completely lost. (L) Results of quantitative analysis of c‐kit‐positive telocyte counts per high‐power field in the corneal stroma of healthy controls (n = 6) and patients with keratoconus (n = 6). Data are mean ± S.D. *P < 0.001 versus control. TC: telocytes; hpf: high‐power field. Scale bar: 100 μm (A and B), 50 μm (D–K). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28714595), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence Representative light and fluorescence microscopy photomicrographs of normal and keratoconic human corneal sections. (A and B) CD34 immunoperoxidase‐based immunohistochemistry with haematoxylin counterstain. (A) In control normal corneas, CD34‐positive stromal cells displaying morphological features of telocytes are orderly arranged and parallel to the corneal surface. (B) In keratoconus, a patchy loss of CD34‐positive stromal cells is mostly evident in the anterior corneal stroma. Insets: Higher magnification views of CD34‐positive corneal stromal cells. (C) Results of quantitative analysis of CD34‐positive telocyte counts per high‐power field in the corneal stroma of healthy controls (n = 6) and patients with keratoconus (n = 6). Data are mean ± S.D. *P < 0.001 versus control. (D–I) Double immunofluorescence labelling for CD34 (red) and PDGFR alpha (green) with DAPI (blue) counterstain for nuclei. (D–F) In control normal corneas, CD34+/PDGFR alpha + stromal cells (telocytes) are orderly distributed throughout the stromal compartment. (G–I) In keratoconic corneas, a patchy loss of CD34+/PDGFR alpha + stromal cells (telocytes) is mainly evident in the subepithelial stroma. (J and K) Double immunofluorescence labelling for CD34 (red) and c‐kit (green) with DAPI (blue) counterstain for nuclei. (J) In control normal corneas, numerous CD34+/c‐kit+ stromal cells (telocytes) are present throughout the stromal layer. (K) In keratoconic corneas, the CD34+/c‐kit+ stromal cell subpopulation is almost completely lost. (L) Results of quantitative analysis of c‐kit‐positive telocyte counts per high‐power field in the corneal stroma of healthy controls (n = 6) and patients with keratoconus (n = 6). Data are mean ± S.D. *P < 0.001 versus control. TC: telocytes; hpf: high‐power field. Scale bar: 100 μm (A and B), 50 μm (D–K). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28714595), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence Representative light and fluorescence microscopy photomicrographs of normal and keratoconic human corneal sections. (A and B) CD34 immunoperoxidase‐based immunohistochemistry with haematoxylin counterstain. (A) In control normal corneas, CD34‐positive stromal cells displaying morphological features of telocytes are orderly arranged and parallel to the corneal surface. (B) In keratoconus, a patchy loss of CD34‐positive stromal cells is mostly evident in the anterior corneal stroma. Insets: Higher magnification views of CD34‐positive corneal stromal cells. (C) Results of quantitative analysis of CD34‐positive telocyte counts per high‐power field in the corneal stroma of healthy controls (n = 6) and patients with keratoconus (n = 6). Data are mean ± S.D. *P < 0.001 versus control. (D–I) Double immunofluorescence labelling for CD34 (red) and PDGFR alpha (green) with DAPI (blue) counterstain for nuclei. (D–F) In control normal corneas, CD34+/PDGFR alpha + stromal cells (telocytes) are orderly distributed throughout the stromal compartment. (G–I) In keratoconic corneas, a patchy loss of CD34+/PDGFR alpha + stromal cells (telocytes) is mainly evident in the subepithelial stroma. (J and K) Double immunofluorescence labelling for CD34 (red) and c‐kit (green) with DAPI (blue) counterstain for nuclei. (J) In control normal corneas, numerous CD34+/c‐kit+ stromal cells (telocytes) are present throughout the stromal layer. (K) In keratoconic corneas, the CD34+/c‐kit+ stromal cell subpopulation is almost completely lost. (L) Results of quantitative analysis of c‐kit‐positive telocyte counts per high‐power field in the corneal stroma of healthy controls (n = 6) and patients with keratoconus (n = 6). Data are mean ± S.D. *P < 0.001 versus control. TC: telocytes; hpf: high‐power field. Scale bar: 100 μm (A and B), 50 μm (D–K). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28714595), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence Representative light and fluorescence microscopy photomicrographs of normal human corneal sections. (A–C) CD34 immunoperoxidase‐based immunohistochemistry with haematoxylin counterstain. (A) CD34‐positive stromal cells are orderly arranged and parallel to the corneal surface. (B and C) At higher magnification, the CD34‐positive stromal cells appear as spindle‐shaped cells with a small oval body and typically two long and thin moniliform cell processes characterized by the alternation of slender segments (arrows) and knobs/dilations (arrowheads) along their length. (D–I) Double immunofluorescence labelling for CD34 (red) and PDGFR alpha (green) with DAPI (blue) counterstain for nuclei. Colocalization of CD34 and PDGFR alpha in stromal cells gives rise to yellow staining either in the anterior corneal stroma (D–F) or in the deeper corneal stromal layer (G–I). CD34+/PDGFR alpha + stromal cells display cell morphologies very evocative for telocytes: a small cell body with very long prolongations (telopodes) characterized by a moniliform silhouette with the alternation of podoms (arrowheads) and podomers (arrows). (J–L) Double immunofluorescence labelling for CD34 (red) and c‐kit (green) with DAPI (blue) counterstain for nuclei. Either in the subepithelial corneal stroma or in the deeper stromal layer, numerous CD34‐positive stromal cells coexpress c‐kit. Inset: Higher magnification view of CD34+/c‐kit+ corneal stromal cells. Scale bar: 50 μm (A, D–F and J–L), 25 μm (B, C and G–I). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28714595), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence Representative light and fluorescence microscopy photomicrographs of normal and keratoconic human corneal sections. (A and B) CD34 immunoperoxidase‐based immunohistochemistry with haematoxylin counterstain. (A) In control normal corneas, CD34‐positive stromal cells displaying morphological features of telocytes are orderly arranged and parallel to the corneal surface. (B) In keratoconus, a patchy loss of CD34‐positive stromal cells is mostly evident in the anterior corneal stroma. Insets: Higher magnification views of CD34‐positive corneal stromal cells. (C) Results of quantitative analysis of CD34‐positive telocyte counts per high‐power field in the corneal stroma of healthy controls (n = 6) and patients with keratoconus (n = 6). Data are mean ± S.D. *P < 0.001 versus control. (D–I) Double immunofluorescence labelling for CD34 (red) and PDGFR alpha (green) with DAPI (blue) counterstain for nuclei. (D–F) In control normal corneas, CD34+/PDGFR alpha + stromal cells (telocytes) are orderly distributed throughout the stromal compartment. (G–I) In keratoconic corneas, a patchy loss of CD34+/PDGFR alpha + stromal cells (telocytes) is mainly evident in the subepithelial stroma. (J and K) Double immunofluorescence labelling for CD34 (red) and c‐kit (green) with DAPI (blue) counterstain for nuclei. (J) In control normal corneas, numerous CD34+/c‐kit+ stromal cells (telocytes) are present throughout the stromal layer. (K) In keratoconic corneas, the CD34+/c‐kit+ stromal cell subpopulation is almost completely lost. (L) Results of quantitative analysis of c‐kit‐positive telocyte counts per high‐power field in the corneal stroma of healthy controls (n = 6) and patients with keratoconus (n = 6). Data are mean ± S.D. *P < 0.001 versus control. TC: telocytes; hpf: high‐power field. Scale bar: 100 μm (A and B), 50 μm (D–K). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28714595), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence Representative light and fluorescence microscopy photomicrographs of normal and keratoconic human corneal sections. (A and B) CD34 immunoperoxidase‐based immunohistochemistry with haematoxylin counterstain. (A) In control normal corneas, CD34‐positive stromal cells displaying morphological features of telocytes are orderly arranged and parallel to the corneal surface. (B) In keratoconus, a patchy loss of CD34‐positive stromal cells is mostly evident in the anterior corneal stroma. Insets: Higher magnification views of CD34‐positive corneal stromal cells. (C) Results of quantitative analysis of CD34‐positive telocyte counts per high‐power field in the corneal stroma of healthy controls (n = 6) and patients with keratoconus (n = 6). Data are mean ± S.D. *P < 0.001 versus control. (D–I) Double immunofluorescence labelling for CD34 (red) and PDGFR alpha (green) with DAPI (blue) counterstain for nuclei. (D–F) In control normal corneas, CD34+/PDGFR alpha + stromal cells (telocytes) are orderly distributed throughout the stromal compartment. (G–I) In keratoconic corneas, a patchy loss of CD34+/PDGFR alpha + stromal cells (telocytes) is mainly evident in the subepithelial stroma. (J and K) Double immunofluorescence labelling for CD34 (red) and c‐kit (green) with DAPI (blue) counterstain for nuclei. (J) In control normal corneas, numerous CD34+/c‐kit+ stromal cells (telocytes) are present throughout the stromal layer. (K) In keratoconic corneas, the CD34+/c‐kit+ stromal cell subpopulation is almost completely lost. (L) Results of quantitative analysis of c‐kit‐positive telocyte counts per high‐power field in the corneal stroma of healthy controls (n = 6) and patients with keratoconus (n = 6). Data are mean ± S.D. *P < 0.001 versus control. TC: telocytes; hpf: high‐power field. Scale bar: 100 μm (A and B), 50 μm (D–K). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28714595), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence Representative light and fluorescence microscopy photomicrographs of normal human corneal sections. (A–C) CD34 immunoperoxidase‐based immunohistochemistry with haematoxylin counterstain. (A) CD34‐positive stromal cells are orderly arranged and parallel to the corneal surface. (B and C) At higher magnification, the CD34‐positive stromal cells appear as spindle‐shaped cells with a small oval body and typically two long and thin moniliform cell processes characterized by the alternation of slender segments (arrows) and knobs/dilations (arrowheads) along their length. (D–I) Double immunofluorescence labelling for CD34 (red) and PDGFR alpha (green) with DAPI (blue) counterstain for nuclei. Colocalization of CD34 and PDGFR alpha in stromal cells gives rise to yellow staining either in the anterior corneal stroma (D–F) or in the deeper corneal stromal layer (G–I). CD34+/PDGFR alpha + stromal cells display cell morphologies very evocative for telocytes: a small cell body with very long prolongations (telopodes) characterized by a moniliform silhouette with the alternation of podoms (arrowheads) and podomers (arrows). (J–L) Double immunofluorescence labelling for CD34 (red) and c‐kit (green) with DAPI (blue) counterstain for nuclei. Either in the subepithelial corneal stroma or in the deeper stromal layer, numerous CD34‐positive stromal cells coexpress c‐kit. Inset: Higher magnification view of CD34+/c‐kit+ corneal stromal cells. Scale bar: 50 μm (A, D–F and J–L), 25 μm (B, C and G–I). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28714595), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence Representative light and fluorescence microscopy photomicrographs of normal human corneal sections. (A–C) CD34 immunoperoxidase‐based immunohistochemistry with haematoxylin counterstain. (A) CD34‐positive stromal cells are orderly arranged and parallel to the corneal surface. (B and C) At higher magnification, the CD34‐positive stromal cells appear as spindle‐shaped cells with a small oval body and typically two long and thin moniliform cell processes characterized by the alternation of slender segments (arrows) and knobs/dilations (arrowheads) along their length. (D–I) Double immunofluorescence labelling for CD34 (red) and PDGFR alpha (green) with DAPI (blue) counterstain for nuclei. Colocalization of CD34 and PDGFR alpha in stromal cells gives rise to yellow staining either in the anterior corneal stroma (D–F) or in the deeper corneal stromal layer (G–I). CD34+/PDGFR alpha + stromal cells display cell morphologies very evocative for telocytes: a small cell body with very long prolongations (telopodes) characterized by a moniliform silhouette with the alternation of podoms (arrowheads) and podomers (arrows). (J–L) Double immunofluorescence labelling for CD34 (red) and c‐kit (green) with DAPI (blue) counterstain for nuclei. Either in the subepithelial corneal stroma or in the deeper stromal layer, numerous CD34‐positive stromal cells coexpress c‐kit. Inset: Higher magnification view of CD34+/c‐kit+ corneal stromal cells. Scale bar: 50 μm (A, D–F and J–L), 25 μm (B, C and G–I). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28714595), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence Representative light and fluorescence microscopy photomicrographs of normal human corneal sections. (A–C) CD34 immunoperoxidase‐based immunohistochemistry with haematoxylin counterstain. (A) CD34‐positive stromal cells are orderly arranged and parallel to the corneal surface. (B and C) At higher magnification, the CD34‐positive stromal cells appear as spindle‐shaped cells with a small oval body and typically two long and thin moniliform cell processes characterized by the alternation of slender segments (arrows) and knobs/dilations (arrowheads) along their length. (D–I) Double immunofluorescence labelling for CD34 (red) and PDGFR alpha (green) with DAPI (blue) counterstain for nuclei. Colocalization of CD34 and PDGFR alpha in stromal cells gives rise to yellow staining either in the anterior corneal stroma (D–F) or in the deeper corneal stromal layer (G–I). CD34+/PDGFR alpha + stromal cells display cell morphologies very evocative for telocytes: a small cell body with very long prolongations (telopodes) characterized by a moniliform silhouette with the alternation of podoms (arrowheads) and podomers (arrows). (J–L) Double immunofluorescence labelling for CD34 (red) and c‐kit (green) with DAPI (blue) counterstain for nuclei. Either in the subepithelial corneal stroma or in the deeper stromal layer, numerous CD34‐positive stromal cells coexpress c‐kit. Inset: Higher magnification view of CD34+/c‐kit+ corneal stromal cells. Scale bar: 50 μm (A, D–F and J–L), 25 μm (B, C and G–I). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28714595), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence Representative light and fluorescence microscopy photomicrographs of normal human corneal sections. (A–C) CD34 immunoperoxidase‐based immunohistochemistry with haematoxylin counterstain. (A) CD34‐positive stromal cells are orderly arranged and parallel to the corneal surface. (B and C) At higher magnification, the CD34‐positive stromal cells appear as spindle‐shaped cells with a small oval body and typically two long and thin moniliform cell processes characterized by the alternation of slender segments (arrows) and knobs/dilations (arrowheads) along their length. (D–I) Double immunofluorescence labelling for CD34 (red) and PDGFR alpha (green) with DAPI (blue) counterstain for nuclei. Colocalization of CD34 and PDGFR alpha in stromal cells gives rise to yellow staining either in the anterior corneal stroma (D–F) or in the deeper corneal stromal layer (G–I). CD34+/PDGFR alpha + stromal cells display cell morphologies very evocative for telocytes: a small cell body with very long prolongations (telopodes) characterized by a moniliform silhouette with the alternation of podoms (arrowheads) and podomers (arrows). (J–L) Double immunofluorescence labelling for CD34 (red) and c‐kit (green) with DAPI (blue) counterstain for nuclei. Either in the subepithelial corneal stroma or in the deeper stromal layer, numerous CD34‐positive stromal cells coexpress c‐kit. Inset: Higher magnification view of CD34+/c‐kit+ corneal stromal cells. Scale bar: 50 μm (A, D–F and J–L), 25 μm (B, C and G–I). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28714595), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human PDGFR alpha by Immunocytochemistry/Immunofluorescence Representative light and fluorescence microscopy photomicrographs of normal and keratoconic human corneal sections. (A and B) CD34 immunoperoxidase‐based immunohistochemistry with haematoxylin counterstain. (A) In control normal corneas, CD34‐positive stromal cells displaying morphological features of telocytes are orderly arranged and parallel to the corneal surface. (B) In keratoconus, a patchy loss of CD34‐positive stromal cells is mostly evident in the anterior corneal stroma. Insets: Higher magnification views of CD34‐positive corneal stromal cells. (C) Results of quantitative analysis of CD34‐positive telocyte counts per high‐power field in the corneal stroma of healthy controls (n = 6) and patients with keratoconus (n = 6). Data are mean ± S.D. *P < 0.001 versus control. (D–I) Double immunofluorescence labelling for CD34 (red) and PDGFR alpha (green) with DAPI (blue) counterstain for nuclei. (D–F) In control normal corneas, CD34+/PDGFR alpha + stromal cells (telocytes) are orderly distributed throughout the stromal compartment. (G–I) In keratoconic corneas, a patchy loss of CD34+/PDGFR alpha + stromal cells (telocytes) is mainly evident in the subepithelial stroma. (J and K) Double immunofluorescence labelling for CD34 (red) and c‐kit (green) with DAPI (blue) counterstain for nuclei. (J) In control normal corneas, numerous CD34+/c‐kit+ stromal cells (telocytes) are present throughout the stromal layer. (K) In keratoconic corneas, the CD34+/c‐kit+ stromal cell subpopulation is almost completely lost. (L) Results of quantitative analysis of c‐kit‐positive telocyte counts per high‐power field in the corneal stroma of healthy controls (n = 6) and patients with keratoconus (n = 6). Data are mean ± S.D. *P < 0.001 versus control. TC: telocytes; hpf: high‐power field. Scale bar: 100 μm (A and B), 50 μm (D–K). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28714595), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF R alpha

PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF‑AA, PDGF‑BB, and PDGF‑AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB and -AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell, and liver endothelial cells. Soluble PDGF R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).

References
  1. Heldin, C.H. and L. Claesson-Welsh (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A. (ed) Oxford University Press, New York, NY p. 202.
Long Name
Platelet-derived Growth Factor Receptor alpha
Entrez Gene IDs
5156 (Human); 18595 (Mouse)
Alternate Names
alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA

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Citations for Human PDGF R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

57 Citations: Showing 1 - 10
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  1. Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease.
    Authors: Kinchen J, Chen HH, Parikh K et al.
    Cell
  2. PDGF Receptor-alpha Does Not Promote HCMV Entry into Epithelial and Endothelial Cells but Increased Quantities Stimulate Entry by an Abnormal Pathway
    Authors: Vanarsdall AL, Wisner TW, Lei H et al.
    PLoS Pathog
  3. Platelet-derived growth factor receptor-alpha is essential for cardiac fibroblast survival
    Authors: Malina J. Ivey, Jill T. Kuwabara, Kara L. Riggsbee, Michelle D. Tallquist
    American Journal of Physiology-Heart and Circulatory Physiology
  4. High-throughput screening for myelination promoting compounds using human stem cell-derived oligodendrocyte progenitor cells
    Authors: Weifeng Li, Cynthia Berlinicke, Yinyin Huang, Stefanie Giera, Anna G. McGrath, Weixiang Fang et al.
    iScience
  5. A human autoimmune organoid model reveals IL-7 function in coeliac disease
    Authors: Santos, AJM;van Unen, V;Lin, Z;Chirieleison, SM;Ha, N;Batish, A;Chan, JE;Cedano, J;Zhang, ET;Mu, Q;Guh-Siesel, A;Tomaske, M;Colburg, D;Varma, S;Choi, SS;Christophersen, A;Baghdasaryan, A;Yost, KE;Karlsson, K;Ha, A;Li, J;Dai, H;Sellers, ZM;Chang, HY;Dunn, JCY;Zhang, BM;Mellins, ED;Sollid, LM;Fernandez-Becker, NQ;Davis, MM;Kuo, CJ;
    Nature
    Species: Human
    Sample Types: Organoid
    Applications: Immunohistochemistry
  6. Harnessing developmental dynamics of spinal cord extracellular matrix improves regenerative potential of spinal cord organoids
    Authors: Sun, Z;Chen, Z;Yin, M;Wu, X;Guo, B;Cheng, X;Quan, R;Sun, Y;Zhang, Q;Fan, Y;Jin, C;Yin, Y;Hou, X;Liu, W;Shu, M;Xue, X;Shi, Y;Chen, B;Xiao, Z;Dai, J;Zhao, Y;
    Cell stem cell
    Species: Rabbit, Rat
    Sample Types: Organoid
    Applications: Immunohistochemistry
  7. Patterning and folding of intestinal villi by active mesenchymal dewetting
    Authors: Huycke, TR;Miyazaki, H;Häkkinen, TJ;Srivastava, V;Barruet, E;McGinnis, CS;Kalantari, A;Cornwall-Scoones, J;Vaka, D;Zhu, Q;Jo, H;DeGrado, WF;Thomson, M;Garikipati, K;Boffelli, D;Klein, OD;Gartner, ZJ;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  8. Metformin promotes Schwann cell remyelination, preserves neural tissue and improves functional recovery after spinal cord injury
    Authors: Huang, Z;Lin, J;Jiang, H;Lin, W;Huang, Z;Chen, J;Xiao, W;Lin, Q;Wang, J;Wen, S;Zhu, Q;Liu, J;
    Neuropeptides
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Non-myogenic mesenchymal cells contribute to muscle degeneration in facioscapulohumeral muscular dystrophy patients
    Authors: L Di Pietro, F Giacalone, E Ragozzino, V Saccone, F Tiberio, M De Bardi, M Picozza, G Borsellino, W Lattanzi, E Guadagni, S Bortolani, G Tasca, E Ricci, O Parolini
    Cell Death & Disease, 2022-09-16;13(9):793.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. Oviductal Oxygen Homeostasis in Patients with Uterine Myoma: Correlation between Hypoxia and Telocytes
    Authors: A Wrona, V Aleksandro, T Bereza, P Basta, A Gil, M Ulatowska-, M Mazur-Lask, K Pity?ski, K Gil
    International Journal of Molecular Sciences, 2022-05-31;23(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Morphologic evidence of telocytes in human thyroid stromal tissue
    Authors: I Rosa, L Ibba-Manne, D Guasti, G Perigli, MS Faussone-P, M Manetti
    Journal of Cellular and Molecular Medicine, 2022-03-20;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Developmental Origins of Human Cortical Oligodendrocytes and Astrocytes
    Authors: Lin Yang, Zhenmeiyu Li, Guoping Liu, Xiaosu Li, Zhengang Yang
    Neuroscience Bulletin
  13. Deciphering the spatial-temporal transcriptional landscape of human hypothalamus development
    Authors: X Zhou, Y Lu, F Zhao, J Dong, W Ma, S Zhong, M Wang, B Wang, Y Zhao, Y Shi, Q Ma, T Lu, J Zhang, X Wang, Q Wu
    Cell Stem Cell, 2021-12-07;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  14. Human skeletal muscle CD90+ fibro-adipogenic progenitors are associated with muscle degeneration in type 2 diabetic patients
    Authors: Jean Farup, Jesper Just, Frank de Paoli, Lin Lin, Jonas Brorson Jensen, Tine Billeskov et al.
    Cell Metabolism
  15. IL-1-driven stromal-neutrophil interactions define a subset of patients with inflammatory bowel disease that does not respond to therapies
    Authors: M Friedrich, M Pohin, MA Jackson, I Korsunsky, SJ Bullers, K Rue-Albrec, Z Christofor, D Sathananth, T Thomas, R Ravindran, R Tandon, RS Peres, H Sharpe, K Wei, GFM Watts, EH Mann, A Geremia, M Attar, Oxford IBD, Roche Fibr, S McCuaig, L Thomas, E Collantes, HH Uhlig, SN Sansom, A Easton, S Raychaudhu, SP Travis, FM Powrie
    Nature Medicine, 2021-10-21;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  16. Oviductal Telocytes in Patients with Uterine Myoma
    Authors: V Aleksandro, A Wrona, T Bereza, K Pity?ski, K Gil
    Biomedicines, 2021-08-20;9(8):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  17. Single-nucleus chromatin accessibility and transcriptomic characterization of Alzheimer's disease
    Authors: S Morabito, E Miyoshi, N Michael, S Shahin, AC Martini, E Head, J Silva, K Leavy, M Perez-Rose, V Swarup
    Nature Genetics, 2021-07-08;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  18. CD109-GP130 interaction drives glioblastoma stem cell plasticity and chemoresistance through STAT3 activity
    Authors: P Filppu, JT Ramanathan, KJ Granberg, E Gucciardo, H Haapasalo, K Lehti, M Nykter, V Le Joncour, P Laakkonen
    JCI Insight, 2021-05-10;6(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. Heterogeneity of glial progenitor cells during the neurogenesis-to-gliogenesis switch in the developing human cerebral cortex
    Authors: Y Fu, M Yang, H Yu, Y Wang, X Wu, J Yong, Y Mao, Y Cui, X Fan, L Wen, J Qiao, F Tang
    Cell Reports, 2021-03-02;34(9):108788.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  20. Histone H3.3G34-Mutant Interneuron Progenitors Co-opt PDGFRA for Gliomagenesis
    Authors: Carol C.L. Chen, Shriya Deshmukh, Selin Jessa, Djihad Hadjadj, Véronique Lisi, Augusto Faria Andrade et al.
    Cell
  21. Origins and Proliferative States of Human Oligodendrocyte Precursor Cells
    Authors: Wei Huang, Aparna Bhaduri, Dmitry Velmeshev, Shaohui Wang, Li Wang, Catherine A. Rottkamp et al.
    Cell
  22. Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles
    Authors: J Just, Y Yan, J Farup, P Sieljacks, M Sloth, M Venø, T Gu, FV de Paoli, JR Nyengaard, R Bæk, MM Jørgensen, J Kjems, K Vissing, KR Drasbek
    Sci Rep, 2020-04-03;10(1):5835.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. Differential Expression of PDGF Receptor-alpha in Human Placental Trophoblasts Leads to Different Entry Pathways by Human Cytomegalovirus Strains
    Authors: Zin Naing, Stuart T. Hamilton, Wendy J. van Zuylen, Gillian M. Scott, William D. Rawlinson
    Scientific Reports
  24. Patch repair of deep wounds by mobilized fascia
    Authors: D Correa-Gal, D Jiang, S Christ, P Ramesh, H Ye, J Wannemache, S Kalgudde G, Q Yu, M Aichler, A Walch, U Mirastschi, T Volz, Y Rinkevich
    Nature, 2019-11-27;0(0):.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  25. Identification of PDGFR alpha + cells in uterine fibroids – link between angiogenesis and uterine telocytes
    Authors: Veronika Aleksandrovych, Tomasz Bereza, Magdalena Ulatowska-Białas, Artur Pasternak, Jerzy A. A. Walocha, Kazimierz Pityński et al.
    Archives of Medical Science
  26. An Immunohistochemical Study of Gastric Mucosa and Critical Review Indicate That the Subepithelial Telocytes Are Prelymphatic Endothelial Cells
    Authors: Oana D. Toader, Mugurel C. Rusu, Laurenţiu Mogoantă, Sorin Hostiuc, Adelina Maria Jianu, Adrian Cosmin Ilie
    Medicina (Kaunas)
  27. The Autonomic Innervation and Uterine Telocyte Interplay in Leiomyoma Formation
    Authors: Veronika Aleksandrovych, Magdalena Kurnik-Łucka, Tomasz Bereza, Magdalena Białas, Artur Pasternak, Dragos Cretoiu et al.
    Cell Transplantation
  28. Anterior Cruciate Ligament Tear Promotes Skeletal Muscle Myostatin Expression, Fibrogenic Cell Expansion, and a Decline in Muscle Quality
    Authors: Bailey D. Peck, Camille R. Brightwell, Darren L. Johnson, Mary Lloyd Ireland, Brian Noehren, Christopher S. Fry
    The American Journal of Sports Medicine
  29. Induction of nuclear protein-1 by thyroid hormone enhances platelet-derived growth factor A mediated angiogenesis in liver cancer
    Authors: CY Chen, SM Wu, YH Lin, HC Chi, SL Lin, CT Yeh, WY Chuang, KH Lin
    Theranostics, 2019-04-13;9(8):2361-2379.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  30. Telocytes constitute a widespread interstitial meshwork in the lamina propria and underlying striated muscle of human tongue
    Authors: Irene Rosa, Cecilia Taverna, Luca Novelli, Mirca Marini, Lidia Ibba-Manneschi, Mirko Manetti
    Scientific Reports
  31. Skeletal muscle fibrosis is associated with decreased muscle inflammation and weakness in patients with chronic kidney disease
    Authors: Matthew K. Abramowitz, William Paredes, Kehao Zhang, Camille R. Brightwell, Julia N. Newsom, Hyok-Joon Kwon et al.
    American Journal of Physiology-Renal Physiology
  32. Locomotor recovery following contusive spinal cord injury does not require oligodendrocyte remyelination
    Authors: Greg J. Duncan, Sohrab B. Manesh, Brett J. Hilton, Peggy Assinck, Jie Liu, Aaron Moulson et al.
    Nature Communications
  33. Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations
    Authors: Christina Philippeos, Stephanie B. Telerman, Bénédicte Oulès, Angela O. Pisco, Tanya J. Shaw, Raul Elgueta et al.
    Journal of Investigative Dermatology
  34. Morphological evidence of telocytes in human synovium
    Authors: I Rosa, M Marini, D Guasti, L Ibba-Manne, M Manetti
    Sci Rep, 2018-02-26;8(1):3581.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  35. Transcriptome analysis of PDGFR?+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFR?+ cell hyperplasia
    Authors: SE Ha, MY Lee, M Kurahashi, L Wei, BG Jorgensen, C Park, PJ Park, D Redelman, KC Sasse, LS Becker, KM Sanders, S Ro
    PLoS ONE, 2017-08-14;12(8):e0182265.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  36. Adaptive changes of telocytes in the urinary bladder of patients affected by neurogenic detrusor overactivity
    Authors: C Traini, MS Fausssone-, D Guasti, G Del Popolo, J Frizzi, S Serni, MG Vannucchi
    J. Cell. Mol. Med., 2017-08-07;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  37. Telocytes in normal and keratoconic human cornea: an immunohistochemical and transmission electron microscopy study
    Authors: M Marini, R Mencucci, I Rosa, E Favuzza, D Guasti, L Ibba-Manne, M Manetti
    J. Cell. Mol. Med., 2017-07-17;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  38. Non-glycanated Decorin Is a Drug Target on Human Adipose Stromal Cells
    Authors: AC Daquinag, A Dadbin, B Snyder, X Wang, AA Sahin, NT Ueno, MG Kolonin
    Mol Ther Oncolytics, 2017-05-17;6(0):1-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  39. ACL injury reduces satellite cell abundance and promotes fibrogenic cell expansion within skeletal muscle
    Authors: Christopher S Fry
    J. Orthop. Res, 2017-01-15;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  40. Telocytes in minor salivary glands of primary Sjögren's syndrome: association with the extent of inflammation and ectopic lymphoid neogenesis
    Authors: Alessia Alunno, Lidia Ibba-Manneschi, Onelia Bistoni, Irene Rosa, Sara Caterbi, Roberto Gerli et al.
    Journal of Cellular and Molecular Medicine
  41. Telocytes subtypes in human urinary bladder
    Authors: Maria‐Giuliana Vannucchi, Chiara Traini, Daniele Guasti, Del Popolo Del Popolo, Maria‐Simonetta Faussone‐Pellegrini
    Journal of Cellular and Molecular Medicine
  42. Identification and characterization of PDGFRalpha+ mesenchymal progenitors in human skeletal muscle.
    Authors: Uezumi A, Fukada S, Yamamoto N, Ikemoto-Uezumi M, Nakatani M, Morita M, Yamaguchi A, Yamada H, Nishino I, Hamada Y, Tsuchida K
    Cell Death Dis, 2014-04-17;5(0):e1186.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  43. Isoflurane-induced Apoptosis of Neurons and Oligodendrocytes in the Fetal Rhesus Macaque Brain
    Authors: Catherine E. Creeley, Krikor T. Dikranian, Gregory A. Dissen, Stephen A. Back, John W. Olney, Ansgar M. Brambrink
    Anesthesiology
  44. Alcohol-induced apoptosis of oligodendrocytes in the fetal macaque brain
    Authors: Catherine E Creeley, Krikor T Dikranian, Stephen A Johnson, Nuri B Farber, John W Olney
    Acta Neuropathologica Communications
  45. Telocytes in Crohn's disease.
    Authors: Milia A, Ruffo M, Manetti M, Rosa I, Conte D, Fazi M, Messerini L, Ibba-Manneschi L
    J Cell Mol Med, 2013-11-19;17(12):1525-36.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  46. Telocytes express PDGFR alpha in the human gastrointestinal tract
    Authors: Maria‐Giuliana Vannucchi, Chiara Traini, Mirko Manetti, Lidia Ibba‐Manneschi, Maria‐Simonetta Faussone‐Pellegrini
    Journal of Cellular and Molecular Medicine
  47. Isoflurane-induced apoptosis of oligodendrocytes in the neonatal primate brain
    Authors: Ansgar M. Brambrink, Stephen A. Back, Art Riddle, Xi Gong, Matthew D. Moravec, Gregory A. Dissen et al.
    Annals of Neurology
  48. Arhgef15 promotes retinal angiogenesis by mediating VEGF-induced Cdc42 activation and potentiating RhoJ inactivation in endothelial cells.
    Authors: Kusuhara, Sentaro, Fukushima, Yoko, Fukuhara, Shigetom, Jakt, Lars Mar, Okada, Mitsuhir, Shimizu, Yuri, Hata, Masayuki, Nishida, Kohji, Negi, Akira, Hirashima, Masanori, Mochizuki, Naoki, Nishikawa, Shin-Ich, Uemura, Akiyoshi
    PLoS ONE, 2012-09-21;7(9):e45858.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  49. Platelet-derived growth factor receptor alpha -positive cells in the tunica muscularis of human colon
    Authors: Masaaki Kurahashi, Yasuko Nakano, Grant W. Hennig, Sean M. Ward, Kenton M. Sanders
    Journal of Cellular and Molecular Medicine
  50. Platelet-derived growth factor-alpha receptor activation is required for human cytomegalovirus infection.
    Authors: Soroceanu L, Akhavan A, Cobbs CS
    Nature, 2008-08-13;455(7211):391-5.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  51. Distinctions between fetal and adult human platelet-derived growth factor-responsive neural precursors.
    Authors: Chojnacki A, Kelly JJ, Hader W, Weiss S
    Ann. Neurol., 2008-08-01;64(2):127-42.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  52. Human bone marrow activates the Akt pathway in metastatic prostate cells through transactivation of the alpha-platelet-derived growth factor receptor.
    Authors: Dolloff NG, Russell MR, Loizos N, Fatatis A
    Cancer Res., 2007-01-15;67(2):555-62.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  53. Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells.
    Authors: Faraone D, Aguzzi MS, Ragone G, Russo K, Capogrossi MC, Facchiano A
    Blood, 2005-12-01;107(5):1896-902.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Immunoprecipitation
  54. Platelet-derived growth factor-AA is an essential and autocrine regulator of vascular endothelial growth factor expression in non-small cell lung carcinomas.
    Authors: Shikada Y, Yonemitsu Y, Koga T, Onimaru M, Nakano T, Okano S, Sata S, Nakagawa K, Yoshino I, Maehara Y, Sueishi K
    Cancer Res., 2005-08-15;65(16):7241-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunodepletion, Immunoprecipitation
  55. Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty.
    Authors: Nili N, Cheema AN, Giordano FJ, Barolet AW, Babaei S, Hickey R, Eskandarian MR, Smeets M, Butany J, Pasterkamp G, Strauss BH
    Am. J. Pathol., 2003-09-01;163(3):869-78.
    Species: Rabbit
    Sample Types: Cell Lysates
    Applications: Western Blot
  56. Content and activity of cAMP response element-binding protein regulate platelet-derived growth factor receptor-alpha content in vascular smooth muscles.
    Authors: Watson PA, Vinson C, Nesterova A, Reusch JE
    Endocrinology, 2002-08-01;143(8):2922-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  57. Improved epicardial cardiac fibroblast generation from iPSCs
    Authors: Whitehead AJ, Hocker JD, Ren B, Engler AJ
    Journal of molecular and cellular cardiology

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Human PDGF R alpha Antibody
By Juwen DuBois on 06/13/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Brain tissue,Spinal cord Species: Mouse