Human PDGF-BB Biotinylated Antibody Summary
Ser82-Thr190
Accession # Q6FHE7
Applications
Human PDGF-BB Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDGF-BB
Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.
Product Datasheets
Citations for Human PDGF-BB Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Transforming Growth Factor-Beta Produced by Non-small Cell Lung Cancer Cells Contributes to Lung Fibroblast Contractile Phenotype
Authors: N Kanaji, M Yokohira, N Watanabe, N Kadowaki, S Bandoh
Anticancer Res., 2018-04-01;38(4):2007-2014.
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Vascular endothelial growth factor signals through platelet-derived growth factor receptor beta in meningiomas in vitro.
Br. J. Cancer, 2012-10-09;107(10):1702-13.
Species: Human
Sample Types: Cell Lysates
Applications: TaqMan Protein Assay -
A molecular and functional analysis of large granular lymphocyte expansions in patients with chronic myelogenous leukemia treated with tyrosine kinase inhibitors
Authors: John J. Powers, Jason A. Dubovsky, P. K. Epling-Burnette, Lynn Moscinski, Ling Zhang, Satu Mustjoki et al.
Leukemia & Lymphoma
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Platelet concentrates: effects of calcium and thrombin on endothelial cell proliferation and growth factor release.
Authors: Lacoste E, Martineau I, Gagnon G
J. Periodontol., 2003-10-01;74(10):1498-507.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
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