Home / PDGF-BB / Human PDGF-BB Antibody

Human PDGF-BB Antibody

Catalog #: AF-220-NA
25 Citations Datasheet
Catalog # Availability Size / Price Qty
AF-220-NA
AF-220-SP
Cell Proliferation Induced by PDGF‑BB and Neutralization by Human PDGF-BB Antibody.
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Citations (25)
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Human PDGF-BB Antibody Summary

Species Reactivity
Human
Specificity
Detects PDGF-BB in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PDGF‑BB
Ser82-Thr190
Accession # P01127
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human PDGF-BB (Catalog # 220-BB)
Neutralization
Measured by its ability to neutralize PDGF‑BB-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line [Raines, E.W. et al. (1985) Methods Enzymol. 109:749]. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 10 ng/mL Recombinant Human PDGF‑BB.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by PDGF‑BB and Neutralization by Human PDGF-BB Antibody. View Larger

Cell Proliferation Induced by PDGF‑BB and Neutralization by Human PDGF-BB Antibody. Recombinant Human PDGF-BB (Catalog # 220-BB) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-BB (10 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human PDGF-BB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-220-NA). The ND50 is typically 0.1-0.5 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-BB

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Long Name
Platelet-derived Growth Factor BB
Entrez Gene IDs
5155 (Human); 24628 (Rat)
Alternate Names
c-sis;IBGC5;PDGF2;PDGF-2;PDGFB;Platelet-derived growth factor subunit B;SIS;SSV; PDGFBB; PDGF-BB

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Citations for Human PDGF-BB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Type H vessel/platelet‐derived growth factor receptor beta + perivascular cell disintegration is involved in vascular injury and bone loss in radiation‐induced bone damage
    Authors: Jiayan Li, Xiaodan Chen, Lin Ren, Xijuan Chen, Tong Wu, Yun Wang et al.
    Cell Proliferation
  2. Rodent Models of HAND and Drug Abuse: Exogenous Administration of Viral Protein(s) and Cocaine
    Authors: Honghong Yao, Shilpa Buch
    Journal of Neuroimmune Pharmacology
  3. A dynamic single cell-based framework for digital twins to prioritize disease genes and drug targets
    Authors: Xinxiu Li, Eun Jung Lee, Sandra Lilja, Joseph Loscalzo, Samuel Schäfer, Martin Smelik et al.
    Genome Medicine
  4. Neointimal hyperplasia after carotid transection and anastomosis surgery is associated with degradation of decorin and platelet-derived growth factor signaling
    Authors: Roshan J. D'Cruz, Valerie B. Sampson, Carly A. Askinas, Rebecca A. Scott, Karyn G. Robinson, Claude A. Beaty et al.
    JVS-Vascular Science
  5. Transforming Growth Factor-Beta Produced by Non-small Cell Lung Cancer Cells Contributes to Lung Fibroblast Contractile Phenotype
    Authors: N Kanaji, M Yokohira, N Watanabe, N Kadowaki, S Bandoh
    Anticancer Res., 2018-04-01;38(4):2007-2014.
  6. Endothelial HIF?-PDGF-B to smooth muscle Beclin1 signaling sustains pathological muscularization in pulmonary hypertension
    Authors: Saddouk, FZ;Kuzemczak, AP;Saito, J;Greif, DM;
    JCI insight
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretion
    Authors: GK Annarapu, D Nolfi-Done, M Reynolds, Y Wang, L Kohut, B Zuckerbrau, S Shiva
    Redox Biology, 2021-12-05;48(0):102205.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Dot Blot
  8. DutaFabs are engineered therapeutic Fab fragments that can bind two targets simultaneously
    Authors: R Beckmann, K Jensen, S Fenn, J Speck, K Krause, A Meier, M Röth, S Fauser, R Kimbung, DT Logan, M Steegmaier, H Kettenberg
    Nature Communications, 2021-01-29;12(1):708.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Detection
  9. Harmine enhances type H vessel formation and prevents bone loss in ovariectomized mice
    Authors: J Huang, H Yin, SS Rao, PL Xie, X Cao, T Rao, SY Liu, ZX Wang, J Cao, Y Hu, Y Zhang, J Luo, YJ Tan, ZZ Liu, B Wu, XK Hu, TH Chen, CY Chen, H Xie
    Theranostics, 2018-03-28;8(9):2435-2446.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Treatment of refractory cutaneous ulcers with mixed sheets consisting of peripheral blood mononuclear cells and fibroblasts
    Authors: Koji Ueno
    Sci Rep, 2016-06-22;6(0):28538.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular Carcinoma.
    Authors: Gao Y, Zhou Z, Lu S, Huang X, Zhang C, Jiang R, Yao A, Sun B, Wang X
    Stem Cells, 2016-01-13;34(4):1112-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
    Authors: Smith A, Bowers S, Stratman A, Davis G
    PLoS ONE, 2013-12-31;8(12):e85147.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. VEGF-C regulates lymphangiogenesis and capillary stability by regulation of PDGF-B.
    Authors: Onimaru M, Yonemitsu Y, Fujii T, Tanii M, Nakano T, Nakagawa K, Kohno R, Hasegawa M, Nishikawa S, Sueishi K
    Am. J. Physiol. Heart Circ. Physiol., 2009-09-04;297(5):H1685-96.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Inhibition of anastomotic intimal hyperplasia using a chimeric decoy strategy against NFkappaB and E2F in a rabbit model.
    Authors: Miyake T, Aoki M, Morishita R
    Cardiovasc. Res., 2008-05-31;79(4):706-14.
    Species: Rabbit
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  15. A CXCL5- and bFGF-dependent effect of PDGF-B-activated fibroblasts in promoting trafficking and differentiation of bone marrow-derived mesenchymal stem cells.
    Authors: Nedeau AE, Bauer RJ, Gallagher K, Chen H, Liu ZJ, Velazquez OC
    Exp. Cell Res., 2008-04-25;314(11):2176-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Pancreatic stellate cells: partners in crime with pancreatic cancer cells.
    Authors: Vonlaufen A, Joshi S, Qu C, Phillips PA, Xu Z, Parker NR, Toi CS, Pirola RC, Wilson JS, Goldstein D, Apte MV
    Cancer Res., 2008-04-01;68(7):2085-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  17. Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity.
    Authors: Mahadevan VS, Campbell M, McKeown PP, Bayraktutan U
    Cardiovasc. Res., 2006-06-27;72(1):60-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells.
    Authors: Lee JH, Ryu IH, Kim EK, Lee JE, Hong S, Lee HK
    Invest. Ophthalmol. Vis. Sci., 2006-03-01;47(3):864-72.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
    Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
    J. Cell. Physiol., 2005-04-01;203(1):35-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts.
    Authors: Helantera I, Loginov R, Koskinen P, Tornroth T, Gronhagen-Riska C, Lautenschlager I
    Nephrol. Dial. Transplant., 2005-02-16;20(4):790-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  21. Dual regulation of proliferation and growth arrest in prostatic stromal cells by transforming growth factor-beta1.
    Authors: Zhou W, Park I, Pins M, Kozlowski JM, Jovanovic B, Zhang J, Lee C, Ilio K
    Endocrinology, 2003-07-24;144(10):4280-4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Connective tissue growth factor and its correlation to other growth factors in experimental granulation tissue.
    Authors: Inkinen K, Wolff H, Lindroos P, Ahonen J
    Connect. Tissue Res., 2003-01-01;44(1):19-29.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  23. Identification of Emetine as a Therapeutic Agent for Pulmonary Arterial Hypertension: Novel Effects of an Old Drug
    Authors: Mohammad Abdul Hai Siddique, Kimio Satoh, Ryo Kurosawa, Nobuhiro Kikuchi, Md. Elias-Al-Mamun, Junichi Omura et al.
    Arteriosclerosis, Thrombosis, and Vascular Biology
  24. Discovery of pan-VEGF inhibitory peptides directed to the extracellular ligand-binding domains of the VEGF receptors
    Authors: Jussara S. Michaloski, Alexandre R. Redondo, Leila S. Magalhães, Caio C. Cambui, Ricardo J. Giordano
    Science Advances
  25. Naringenin Inhibits Neointimal Hyperplasia Following Arterial Reconstruction With Interpositional Vein Graft
    Authors: Cenk Cayci, Trevor C. Wahlquist, Serin I. Seckin, Vefa Ozcan, Ayse B. Tekinay, Timothy P. Martens et al.
    Annals of Plastic Surgery

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