Home / PDGF-BB / Human PDGF-BB Antibody

Human PDGF-BB Antibody

Catalog #: AB-220-NA
5 Citations Datasheet
Catalog # Availability Size / Price Qty
AB-220-NA
Cell Proliferation Induced by PDGF‑BB and Neutralization by Human PDGF‑BB Antibody.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PDGF-BB Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDGF-BB in direct ELISAs and Western blots.  In direct ELISAs, approximately 100% cross-reactivity with recombinant human (rh) PDGF-AB is observed, approximately 50% cross-reactivity with recombinant rat PDGF-B and natural porcine PDGF is observed, and less than 10% cross-reactivity with rhPDGF-A is observed. 
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human PDGF‑BB
Ser82-Thr190
Accession # P01127
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human PDGF-BB (Catalog # 220-BB)
Neutralization
Measured by its ability to neutralize PDGF‑BB-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Raines, E. W. et al. (1985) Methods Enzymol. 109:749. The Neutralization Dose (ND50) is typically 0.25-1.5 µg/mL in the presence of 20 ng/mL Recombinant Human PDGF‑BB.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by PDGF‑BB and Neutralization by Human PDGF‑BB Antibody. View Larger

Cell Proliferation Induced by PDGF‑BB and Neutralization by Human PDGF‑BB Antibody. Recombinant Human PDGF-BB (Catalog # 220-BB) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human PDGF-BB (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-BB Polyclonal Antibody (Catalog # AB-220-NA). The ND50 is typically 0.25-1.5 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-BB

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Long Name
Platelet-derived Growth Factor BB
Entrez Gene IDs
5155 (Human); 24628 (Rat)
Alternate Names
c-sis;IBGC5;PDGF2;PDGF-2;PDGFB;Platelet-derived growth factor subunit B;SIS;SSV; PDGFBB; PDGF-BB

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Citations for Human PDGF-BB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis
    Authors: SF Mause, E Ritzel, A Deck, F Vogt, EA Liehn
    International Journal of Molecular Sciences, 2022-01-14;23(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. RASSF1A inhibits PDGFB-driven malignant phenotypes of nasopharyngeal carcinoma cells in a YAP1-dependent manner
    Authors: YY Liang, XB Deng, XT Lin, LL Jiang, XT Huang, ZW Mo, YW Yuan, MT Teh
    Cell Death Dis, 2020-10-14;11(10):855.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Acidic fibroblast growth factor underlies microenvironmental regulation of MYC in pancreatic cancer
    Authors: Sohinee Bhattacharyya, Chet Oon, Aayush Kothari, Wesley Horton, Jason Link, Rosalie C. Sears et al.
    Journal of Experimental Medicine
  4. Long noncoding RNA LERFS negatively regulates rheumatoid synovial aggression and proliferation
    Authors: Y Zou, S Xu, Y Xiao, Q Qiu, M Shi, J Wang, L Liang, Z Zhan, X Yang, N Olsen, SG Zheng, H Xu
    J. Clin. Invest., 2018-09-10;0(0):.
    Species: Human
    Sample Types: Synovial Fluid
    Applications: Neutralization
  5. Hypoxia Differentially Regulates Arterial and Venous Smooth Muscle Cell Migration.
    Authors: Chanakira A, Kir D, Barke R, Santilli S, Ramakrishnan S, Roy S
    PLoS ONE, 2015-09-18;10(9):e0138587.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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