Home / PDGF-B / Human PDGF-B Antibody

Human PDGF-B Antibody

Catalog #: MAB1739
2 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
MAB1739-SP
MAB1739-500
MAB1739-100
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PDGF-B Antibody Summary

Species Reactivity
Human
Specificity
Detects the human PDGF-B subunit in direct ELISAs and Western blots. In Western blots, shows 50-100% cross-reactivity with recombinant human (rh) PDGF‑AB and rrPDGF-BB but no cross-reactivity with rhCTGF, rhPlGF, rhVEGF‑B186, rhVEGF‑C, or rhVEGF‑D.
Source
Monoclonal Mouse IgG1 Clone # 108128
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PDGF‑BB
Ser82-Thr190
Accession # P01127
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human PDGF-B (Catalog # 220-BB)
under non-reducing conditions only

Human PDGF-AB Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human PDGF-AA/AB Biotinylated Antibody (Catalog # BAF221)

Standard: Recombinant Human PDGF-AB Protein, CF (Catalog # 222-AB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-B

Four PDGF family members, A, B, C and D, have been identified. PDGF-B can exist as a disulfide-linked homodimer (PDGF-BB) or heterodimer (PDGF-AB). PDGF-B binds and activates PDGF R alpha and PDGF R beta.

Long Name
Platelet-derived Growth Factor B
Entrez Gene IDs
5155 (Human); 24628 (Rat); 100070283 (Equine)
Alternate Names
B chain; Becaplermin; C-Sis; FLJ12858; IBGC5; PDGF subunit B; PDGF2; PDGF-2; PDGFB; PDGF-B; platelet-derived growth factor 2; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis)oncogene homolog); Platelet-derived growth factor beta polypeptide; platelet-derived growth factor subunit B; platelet-derived growth factor, B chain; Platelet-derived growth factor, beta polypeptide (oncogene SIS); Proto-oncogene c-Sis; SIS; SSV

Product Datasheets

You must select a language.

x
Product Datasheet

Citations for Human PDGF-B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. PDGF beta targeting in cervical cancer cells suggest a fine-tuning of compensatory signalling pathways to sustain tumourigenic stimulation
    Authors: Oana Mihaela Tudoran, Olga Soritau, Loredana Balacescu, Laura Pop, Guillaume Meurice, Simona Visan et al.
    Journal of Cellular and Molecular Medicine
  2. Early transcriptional pattern of angiogenesis induced by EGCG treatment in cervical tumour cells
    Authors: Oana Tudoran, Olga Soritau, Ovidiu Balacescu, Loredana Balacescu, Cornelia Braicu, Meda Rus et al.
    Journal of Cellular and Molecular Medicine

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human PDGF-B Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human PDGF-B Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human PDGF-B Antibody
By Anonymous on 07/19/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human
ELISA Human PDGF-B Antibody MAB1739