Human PDGF Antibody

Catalog #: AB-20-NA
4 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
AB-20-NA

Cell Proliferation Induced by PDGF and Neutralization by Human PDGF Antibody.

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PDGF Antibody Summary

Species Reactivity

Human

Specificity

Detects human PDGF in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) PDGF‑AA, 60% cross-reactivity with rhPDGF-AB, and nearly 100% cross-reactivity with rhPDGF-BB is observed.

Source

Polyclonal Rabbit IgG

Purification

Protein A or G purified

Immunogen

Human platelet-derived PDGF

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Human PDGF (Catalog # 120-HD)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize PDGF-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line [Rizzino, A. et al. (1988) Cancer Res. 48:4266]. The Neutralization Dose (ND₅₀) is typically 5-10 µg/mL in the presence of 10 ng/mL Human PDGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by PDGF and Neutralization by Human PDGF Antibody. Human PDGF (Catalog # 120-HD) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Human PDGF (10 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human PDGF Polyclonal Antibody (Catalog # AB-20-NA). The ND₅₀ is typically 5-10 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF

PDGF was originally discovered as a major mitogenic factor in serum but not in plasma. PDGF is stored in platelet alpha granules and released upon platelet activation. Besides megakaryocytes, other cell types, including endothelial cells, monocyte/macrophages, vascular smooth muscle cells, fibroblasts, cytotrophoblasts and a variety of transformed or neoplastic cells, have been shown to produce PDGF. PDGFs are disulfide-linked dimers. The subunits of the PDGF dimers are homologous polypeptides designated PDGF-A and PDGF-B chains. Natural PDGFs can exist either as homodimers (PDGF-AA, PDGF-BB) or heterodimers (PDGF-AB). Although all three isoforms of PDGF exist in human platelets, R&D Systems hPDGF consists predominantly of hPDGF-AB heterodimers.

Two distinct PDGF receptors, the alpha -receptor and the beta -receptor, have been identified. The two receptors are structurally related, with an extracellular portion containing five immunoglobulin-like domains, a single transmembrane region, and an intracellular portion with a protein-tyrosine kinase domain. The alpha -receptor binds both the A and B chains with high affinity whereas the beta -receptor binds only the B-chain with high affinity. Receptor dimerization is induced upon ligand binding.

In addition to being a potent mitogen for cells of mesenchymal origin, PDGF has also been shown to be a potent chemoattractant for mesenchymal cells, mononuclear cells and neutrophils and has been reported to be important in the modification of cellular matrix constituents.

Long Name

Platelet-derived Growth Factor

Entrez Gene IDs

5154 (Human)

Alternate Names

PDGF; PDGF1; PDGF-1; PDGFA; PDGF-A

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human PDGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Fibroblast response to gadolinium: role for platelet-derived growth factor receptor
Authors: Narasimharao Bhagavathula, Michael K. Dame, Marissa DaSilva, William Jenkins, Muhammad N. Aslam, Patricia Perone et al.
Investigative Radiology
Improvement in disability after alemtuzumab treatment of multiple sclerosis is associated with neuroprotective autoimmunity.
Authors: Jones JL, Anderson JM, Phuah CL, Fox EJ, Selmaj K, Margolin D, Lake SL, Palmer J, Thompson SJ, Wilkins A, Webber DJ, Compston DA, Coles AJ
Brain, 2010-07-21;133(0):2232-47.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
Authors: Villeneuve J, Block A, Le Bousse-Kerdiles MC, Lepreux S, Nurden P, Ripoche J, Nurden AT
Exp. Hematol., 2009-05-03;37(7):849-56.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Temporally expressed PDGF and FGF-2 regulate embryonic coronary artery formation and growth.
Authors: Tomanek RJ, Hansen HK, Christensen LP
Arterioscler. Thromb. Vasc. Biol., 2008-04-17;28(7):1237-43.
Species: Avian
Sample Types: Whole Tissue
Applications: Neutralization