Human Pancreatic Polypeptide/PP Antibody Summary
Ala30-Leu95
Accession # P01298
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Pancreatic Polypeptide/PP in Human Pancreas. Pancreatic Polypeptide/PP was detected in immersion fixed paraffin-embedded sections of human pancreas using Human Pancreatic Polypeptide/PP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6297) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane of islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pancreatic Polypeptide/PP
Pancreatic polypeptide (PP) is an ~4 kDa, unglycosylated member of the Neuropeptide-Y family of secreted peptide hormones. Human PP is synthesized with a 29 amino acid (aa) signal sequence and a 66 aa prohormone that contains the 36 aa PP hormone, a 20 aa icosapeptide of unknown function, and a C-terminal prosequence. PP is produced by pancreatic islet F-cells and released to the circulation following a meal. It slows stomach emptying time and insulin secretion and is thought to inhibit further food intake. The human PP prohormone shares 57% and 55% aa identity with mouse and rat PP, respectively.
Product Datasheets
Citation for Human Pancreatic Polypeptide/PP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Differentiation of stem cell-derived pancreatic progenitors into insulin-secreting islet clusters in a multiwell-based static 3D culture system
Authors: Shenghui Liang, Jia Zhao, Robert K. Baker, Elisa Tran, Lisa Zhan, Timothy J. Kieffer
Cell Reports Methods
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Pancreatic Polypeptide/PP Antibody
There are currently no reviews for this product. Be the first to review Human Pancreatic Polypeptide/PP Antibody and earn rewards!
Have you used Human Pancreatic Polypeptide/PP Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
