Human p53 APC-conjugated Antibody

Catalog #: IC13551A Datasheet

Catalog #	Availability	Size / Price	Qty
IC13551A

Detection of p53 in MCF-7 cells by Flow Cytometry

1 Image

All p53 Antibodies

Product Details

FAQs

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human p53 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human p53 in flow cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 184727

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human p53
Asp7-Asp393
Accession # P04637

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

MCF‑7 human breast cancer cell line fixed and permeabilized with FlowX FoxP3 Fixation and Permeabilization Buffer kit

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow (Intracellular)

View Larger

Detection of p53 in MCF-7 cells by Flow Cytometry MCF-7 cells were stained with Mouse Anti-Human p53 APC‑conjugated Monoclonal Antibody (Catalog # IC13551A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: p53

The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.

Entrez Gene IDs

7157 (Human); 22059 (Mouse); 24842 (Rat)

Alternate Names

Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53