Human OSMR beta Biotinylated Antibody Summary
Glu28-Ser739
Accession # Q99650
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: OSMR beta
OSM R beta is a 150‑180 kDa member of the IL-6 receptor family. It associates with gp130 to form the type II OSM receptor that is responsive to OSM. The gp130 subunit is shared by other IL-6 family cytokine receptors (1, 2, 3, 4), and OSM R beta associates with gp130-like receptor (GPL) to form a receptor complex responsive to IL-31 (5, 6). The human OSM R beta cDNA encodes a 979 amino acid (aa) precursor that includes a 27 aa signal sequence, a 712 aa extracellular domain (ECD), a 22 aa transmembrane segment, and a 218 aa cytoplasmic domain. The ECD contains one partial and one complete hematopoietin domain, an Ig-like domain, and three fibronectin type-III domains. The cytoplasmic domain contains box1, 2, and 3 motifs (7). Within the ECD, human OSM R beta shares 55%, 58%, 61%, and 72% aa sequence identity with mouse, rat, bovine, and canine OSM R beta, respectively. It also shares 31% aa sequence identity with human LIF R, but less than 20% aa sequence identity with human CNTF R alpha, G-CSF R, IL-6 R, IL-11 R alpha, and TCCR. OSM R beta does not bind cytokines directly, but increases the affinity of gp130 for OSM, and GPL for IL-31 (7, 8). OSM R beta, gp130, and GPL each initiate signaling events following ligand stimulation (9, 10). Jak/STAT and MAPK pathways are activated by OSM R beta -containing receptors (9, 11, 12, 13), including STAT5b and SHC which are not activated by other IL-6 family receptors (10, 13). In mice, the loss of OSM R beta expression blocks erythroid progenitor development in bone marrow, and dramatically reduces the number of circulating platelets and erythrocytes (14). The type II OSM receptor is the only IL-6 family receptor that promotes osteoblast differentiation in calvaria cell cultures (15).
- Chen, S.-H. and E.N. Benveniste (2004) Cytokine Growth Factor Rev. 15:379.
- Heinrich, P.C. et al. (2003) Biochem. J. 374:1.
- Tanaka, M. and A. Miyajima (2003) Rev. Physiol. Biochem. Pharmacol. 149:39.
- Gearing, D.P. et al. (1992) Science 255:1434.
- Dillon, S.R. et al. (2004) Nat. Immunol. 5:752.
- Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.
- Mosley, B. et al. (1996) J. Biol. Chem. 271:32635.
- Diveu, C. et al. (2004) Eur. Cytokine Netw. 15:291.
- Dreuw, A. et al. (2004) J. Biol. Chem. 279:36112.
- Wang, Y. et al. (2000) J. Biol. Chem. 275:25273.
- Hermanns, H.M. et al. (2000) J. Biol. Chem. 275:40742.
- Kuropatwinski, K.K. et al. (1997) J. Biol. Chem. 272:15135.
- Auguste, P. et al. (1997) J. Biol. Chem. 272:15760.
- Tanaka, M. et al. (2003) Blood 102:3154.
- Malaval, L. et al. (2005) J. Cell. Physiol. 204:585.
Product Datasheets
Citations for Human OSMR beta Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
2
Citations: Showing 1 - 2
Filter your results:
Filter by:
-
Engineered Interleukin-6-derived cytokines recruit artificial receptor complexes and disclose CNTF signaling via the OSMR
Authors: Rafii, P;Cruz, PR;Ettich, J;Seibel, C;Padrini, G;Wittich, C;Lang, A;Petzsch, P;Köhrer, K;Moll, JM;Floss, DM;Scheller, J;
The Journal of biological chemistry
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot -
Cytokimera GIL-11 rescued IL-6R deficient mice from partial hepatectomy-induced death by signaling via non-natural gp130:LIFR:IL-11R complexes
Authors: Puyan Rafii, Christiane Seibel, Hendrik T. Weitz, Julia Ettich, Anna Rita Minafra, Patrick Petzsch et al.
Communications Biology
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human OSMR beta Biotinylated Antibody
There are currently no reviews for this product. Be the first to review Human OSMR beta Biotinylated Antibody and earn rewards!
Have you used Human OSMR beta Biotinylated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
