Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antibody
Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antibody Summary
Thr176-Lys246
Accession # NP_039250
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by NRG1‑ beta 1/HRG1‑ beta 1 and Neutralization by Human NRG1‑ beta 1/HRG1‑ beta 1 Antibody. Recombinant Human NRG1-beta 1/ HRG1-beta 1 EGF Domain (Catalog # 396-HB) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NRG1-beta 1/ HRG1-beta 1 EGF Domain (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-396-NA). The ND50 is typically 0.5-2.0 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuregulin-1 beta 1/NRG1 beta 1
The neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. Through alternative splicing or the use of alternative promoters, Nrg-1 has been shown to encode more than 14 soluble or transmembrane proteins. The extracellular domain of the transmembrane NRG1 isoforms can be proteolytically cleaved to release soluble growth factors. All NRG1 isoforms contain an EGF‑like domain ( alpha - or beta -splice variant that differ in their C-terminal region) that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. The ErbB3 or ErbB4 subsequently recruits and heterodimerizes with ErbB2, resulting in tyrosine phosphorylation and NRG1 signaling. NRG1 isoforms can be classified into three major subtypes. Type I (Neu Differentiation Factor, NDF; Heregulin, HRG; Acetylcholine Receptor Inducing Activity, ARIA) and type II (Glial Growth Factor, GGF) NRG1s have an immunoglobulin (Ig)-like domain N-terminal to the EGF‑like domain. Type I NRG1s differ from type II NRG1s by having a glycosylation-rich domain between the Ig‑like and the EGF‑like domains. Type III NRG1s (Sensory and Motor neuron-Derived Factor) lacks the Ig‑like domain but has a cysteine rich domain (CRD) instead. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.
- Buonanno, A., and Fischbach, G.D. (2001) Curr. Opin. Neurobiol. 11:287.
- Adlkofer, K. and Lai, C. (2000) Glia 29:104.
- Garratt, A.N. et al. (2000) BioEssays 22:987.
Product Datasheets
Citations for Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Nrg1 beta Released in Remote Ischemic Preconditioning Improves Myocardial Perfusion and Decreases Ischemia/Reperfusion Injury via ErbB2-Mediated Rescue of Endothelial Nitric Oxide Synthase and Abrogation of Trx2 Autophagy
Authors: Venkatesh Kundumani-Sridharan, Jaganathan Subramani, Cade Owens, Kumuda C. Das
Arteriosclerosis, Thrombosis, and Vascular Biology
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Neuregulin-1 is neuroprotective in a rat model of organophosphate-induced delayed neuronal injury
Authors: Yonggang Li, Pamela J. Lein, Cuimei Liu, Donald A. Bruun, Cecilia Giulivi, Gregory D. Ford et al.
Toxicology and Applied Pharmacology
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alpha -Dystroglycan is essential for the induction of Egr3, a transcription factor important in muscle spindle formation
Authors: Stacey Williams, Christian Jacobson
Developmental Neurobiology
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CAN017, a novel anti-HER3 antibody, exerted great potency in mouse avatars of esophageal squamous cell carcinoma with NRG1 as a biomarker
Authors: H Liao, C Zhang, Z Chen, Y Gao, Z Li, L Wang, Y Li, L Shen, J Gao
American journal of cancer research, 2021-04-15;11(4):1697-1708.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Preventive effects of heregulin-beta1 on macrophage foam cell formation and atherosclerosis.
Authors: Xu G, Watanabe T, Iso Y, Koba S, Sakai T, Nagashima M, Arita S, Hongo S, Ota H, Kobayashi Y, Miyazaki A, Hirano T
Circ. Res., 2009-07-30;105(5):500-10.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
Hepatitis C Virus Activates a Neuregulin-Driven Circuit to Modify Surface Expression of Growth Factor Receptors of the ErbB Family
Authors: Sabine Stindt, Patricia Cebula, Ute Albrecht, Verena Keitel, Jan Schulte am Esch, Wolfram T. Knoefel et al.
PLOS ONE
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NRG1 and KITL signal downstream of retinoic acid in the germline to support soma-free syncytial growth of differentiating spermatogonia.
Authors: Chapman KM, Medrano GA, Chaudhary J, Hamra FK.
Cell Death Discov.
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