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Human/Mouse Nrf2 Antibody

Catalog #: AF3925
8 Citations Datasheet
Catalog # Availability Size / Price Qty
AF3925
AF3925-SP
Detection of Human Nrf2 by Western Blot.
2 Images
Product Details
Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Nrf2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Nrf2 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse Nrf2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Nrf2
Met17-Asn605
Accession # Q16236
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2-2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Nrf2 antibody by Western Blot. View Larger

Detection of Human Nrf2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 10 µM MG 132 (Catalog # 1748) for 4 hours. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse Nrf2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3925) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Nrf2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Mouse Nrf2 antibody by Western Blot. View Larger

Detection of Mouse Nrf2 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse Nrf2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3925) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Nrf2 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nrf2

NF-E2 related factor 2 (Nrf2) is a member of the cap ‘n’ collar family of basic leucine zipper transcription factors. By binding antioxidant response elements (AREs), Nrf2 activates the transcription of genes encoding antioxidant and phase II drug-metabolizing enzymes needed to protect cells against oxidative damage. Transcriptional targets regulated by Nrf2 include genes encoding glutathione S-transferases, superoxide dismutase-1, and heme oxygenase-1.

Long Name
NF-E2-related Factor 2
Entrez Gene IDs
4780 (Human); 18024 (Mouse); 83619 (Rat)
Alternate Names
HEBP1; NFE2L2; NF-E2-related factor 2; NFE2-related factor 2; NRF2 erythroid derived 2, like 2; Nrf2; nuclear factor (erythroid-derived 2)-like 2; nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2

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Citations for Human/Mouse Nrf2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Indenopyrene and Blue-Light Co-Exposure Impairs the Tightly Controlled Activation of Xenobiotic Metabolism in Retinal Pigment Epithelial Cells: A Mechanism for Synergistic Toxicity
    Authors: Zinflou, C;Rochette, PJ;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Fraction
    Applications: Western Blot
  2. Effect of Postnatal Epigallocatechin-Gallate Treatment on Cardiac Function in Mice Prenatally Exposed to Alcohol
    Authors: Vicente Andreu-Fernández, Mariona Serra-Delgado, Laura Almeida-Toledano, Àgueda García-Meseguer, Melina Vieiros, Anna Ramos-Triguero et al.
    Antioxidants (Basel)
  3. ARMS-NF-?B signaling regulates intracellular ROS to induce autophagy-associated cell death upon oxidative stress
    Authors: Liao YH, Wu JT, Hsieh IC et al.
    iScience
  4. ARMS-NF-?B signaling regulates intracellular ROS to induce autophagy-associated cell death upon oxidative stress
    Authors: Liao YH, Wu JT, Hsieh IC et al.
    iScience
  5. Antioxidant and Anti-Inflammatory Properties of Rubber Seed Oil in Lipopolysaccharide-Induced RAW 267.4 Macrophages
    Authors: Jing Liu, Lulu Zhao, Hongying Cai, Zitao Zhao, Yongbao Wu, Zhiguo Wen et al.
    Nutrients
  6. Regulation of Human gamma δ T Cells by BTN3A1 Protein Stability and ATP-Binding Cassette Transporters
    Authors: David A. Rhodes, Hung-Chang Chen, James C. Williamson, Alfred Hill, Jack Yuan, Sam Smith et al.
    Frontiers in Immunology
  7. Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles
    Authors: Eejung Kim, Nina Ilic, Yashaswi Shrestha, Lihua Zou, Atanas Kamburov, Cong Zhu et al.
    Cancer Discovery
  8. Patterns of mRNA and protein expression during minus-lens compensation and recovery in tree shrew sclera
    Authors: Hong Gao, Michael R. Frost, John T. Siegwart, Thomas T. Norton
    Mol Vis

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