Human NPY1R APC-conjugated Antibody

Catalog #: FAB6400A Datasheet
Catalog # Availability Size / Price Qty
FAB6400A
Detection of NPY1R in MCF‑7 Human Cell Line by Flow Cytometry.
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Human NPY1R APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human NPY1R in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 556153
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human NPY1R
Met1-Ile384
Accession # P25929
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of NPY1R antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of NPY1R in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human NPY1R APC-conjugated Monoclonal Antibody (Catalog # FAB6400A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: NPY1R

Neuropeptide Y Receptor type 1 (NPY1R) is a 44 kDa (unglycosylated) member of the G protein coupled receptor 1 family. Human NPY1R is 384 amino acids (aa) in length and contains seven transmembrane regions and three potential sites for N-linked glycosylation. In addition, aa 338 is an S-palmitoyl cysteine lipid-binding site and aa 368 is a phosphoserine. Human NPY1R shares 94% and 93% aa sequence identity with mouse and rat NPY1R, respectively. Functionally, NPY1R is a receptor for Neuropeptide Y and Peptide YY.

Long Name
Neuropeptide Y Receptor Y1
Entrez Gene IDs
4886 (Human); 18166 (Mouse)
Alternate Names
neuropeptide Y receptor type 1; neuropeptide Y receptor Y1; NPY1R; NPY1-R; NPYR; NPYY1

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