Human NKp46/NCR1 Antibody
Human NKp46/NCR1 Antibody Summary
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
The ED50 for this effect is typically ≤ 1 μg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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NKp46/NCR1 in NK-92 Human Cell Line and Human PBMCs. NKp46/NCR1 was detected in immersion fixed NK-92 human natural killer lymphoma cell line (left panel, positive stain) and human peripheral blood mononuclear cells (PBMCs; right panel, negative stain) using Mouse Anti-Human NKp46/NCR1 Monoclonal Antibody (Catalog # MAB1850) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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NKp46/NCR1 in Human Tonsil. NKp46/NCR1 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human NKp46/NCR1 Monoclonal Antibody (Catalog # MAB1850) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to natural killer cells in germinal center. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of NKp46/NCR1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human NKp46/NCR1 Monoclonal Antibody (Catalog # MAB1850) or (B) Mouse IgG2B Isotype Control (MAB0041) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Rabbit Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (FAB24086P). Staining was performed using our Staining Membrane-associated Proteins protocol.
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Detection of NKp46/NCR1 in Human Tonsil. Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for NKp46 mRNA (ACD RNAScope Probe, catalog #312658; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human NKp46 monoclonal antibody (R&D Systems catalog # MAB1850) at 15ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cell surface.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKp46/NCR1
NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. The CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is up-regulated by IL-2. NKp46 is a type I transmembrane protein with two extracellular Ig-like domains followed by a short stalk region, a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. Through its positive charge in the transmembrane domain, NKp46 associates with the ITAM‑bearing signal adapter proteins, CD3 zeta and Fc epsilon R1 gamma, which are able to form disulfide-linked homodimers and heterodimers (3, 8). Studies with neutralizing antibodies indicate that the three NCRs are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (4). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (5‑7). Human NKp46 shares 58% and 59% amino acid sequence identity with the mouse and rat proteins, respectively.
- Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
- Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
- Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
- Pende, D. et al. (1999) J. Exp. Med. 190:1505.
- Arnon, T. et al. (2004) Blood 103:664.
- Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
- Mandelboim, O. et al. (2001) Nature 409:1055.
- Moretta, A. et al. (2001) Annu. Rev. Immunol. 19:197.
Product Datasheets
Citations for Human NKp46/NCR1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Expression of human Interferon Regulatory Factor 3 (IRF-3) in alveolar macrophages relates to clinical and functional traits in COPD
Authors: Baraldo, S;Bonato, M;Cassia, S;Casolari, P;De Ferrari, L;Tiné, M;Baraldi, F;Bigoni, T;Riccio, AM;Braido, F;Saetta, M;Papi, A;Contoli, M;
Respiratory research
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Characterization of HIF-1? Knockout Primary Human Natural Killer Cells Including Populations in Allogeneic Glioblastoma
Authors: Nakazawa, T;Morimoto, T;Maeoka, R;Yamada, K;Matsuda, R;Nakamura, M;Nishimura, F;Yamada, S;Park, YS;Tsujimura, T;Nakagawa, I;
International journal of molecular sciences
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
An efficient feeder-free and chemically-defined expansion strategy for highly purified natural killer cells derived from human cord blood
Authors: Nakazawa, T;Maeoka, R;Morimoto, T;Matsuda, R;Nakamura, M;Nishimura, F;Yamada, S;Nakagawa, I;Park, YS;Ito, T;Nakase, H;Tsujimura, T;
Regenerative therapy
Species: Human
Sample Types: Whole Cells
Applications: ELISA Capture -
Suppression of the antitumoral activity of natural killer cells under indirect coculture with cancer-associated fibroblasts in a pancreatic TIME-on-chip model
Authors: Kim, HA;Kim, H;Nam, MK;Park, JK;Lee, MY;Chung, S;Lee, KM;Kuh, HJ;
Cancer cell international
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Sex Differences in the Systemic and Local Immune Response of Pancreatic Cancer Patients
Authors: A Ahmed, S Köhler, R Klotz, N Giese, T Hackert, C Springfeld, D Jäger, N Halama
Cancers, 2023-03-17;15(6):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Human Hematopoietic Stem Cell Engrafted IL-15 Transgenic NSG Mice Support Robust NK Cell Responses and Sustained HIV-1 Infection
Authors: SA Abeynaike, TR Huynh, A Mehmood, T Kim, K Frank, K Gao, C Zalfa, A Gandarilla, L Shultz, S Paust
Viruses, 2023-01-27;15(2):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Cytotoxic CD8+ T cells may be drivers of tissue destruction in Sj�gren's syndrome
Authors: N Kaneko, H Chen, CA Perugino, T Maehara, R Munemura, S Yokomizo, J Sameshima, TJ Diefenbach, KR Premo, A Chinju, Y Miyahara, M Sakamoto, M Moriyama, JH Stone, S Nakamura, S Pillai
Scientific Reports, 2022-09-14;12(1):15427.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
The Combination of Radiotherapy and Complement C3a Inhibition Potentiates Natural Killer cell Functions Against Pancreatic Cancer
Authors: Quaovi H. Sodji, Dhanya K. Nambiar, Vignesh Viswanathan, Rie von Eyben, Deana Colburg, Michael S. Binkley et al.
Cancer Research Communications
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Balanced engagement of activating and inhibitory receptors mitigates human NK cell exhaustion
Authors: JA Myers, D Schirm, L Bendzick, R Hopps, C Selleck, P Hinderlie, M Felices, JS Miller
JCI Insight, 2022-08-08;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Binding Assay -
Establishment of an efficient ex�vivo expansion strategy for human natural killer cells stimulated by defined cytokine cocktail and antibodies against natural killer cell activating receptors
Authors: T Nakazawa, T Morimoto, R Maeoka, R Matsuda, M Nakamura, F Nishimura, S Yamada, I Nakagawa, YS Park, H Nakase, T Tsujimura
Regenerative Therapy, 2022-07-21;21(0):185-191.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Capture -
Radiotherapy orchestrates natural killer cell dependent antitumor immune responses through CXCL8
Authors: T Walle, JA Kraske, B Liao, B Lenoir, C Timke, E von Bohlen, F Tran, P Griebel, D Albrecht, A Ahmed, M Suarez-Car, A Jiménez-Sá, T Beikert, A Tietz-Dahl, AN Menevse, G Schmidt, M Brom, JHW Pahl, W Antonopoul, M Miller, RL Perez, F Bestvater, NA Giese, P Beckhove, P Rosenstiel, D Jäger, O Strobel, D Pe'er, N Halama, J Debus, A Cerwenka, PE Huber
Science Advances, 2022-03-23;8(12):eabh4050.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Flow cytometric single cell-based assay to simultaneously detect cell death, cell cycling, DNA content and cell senescence
Authors: E Lieschke, Z Wang, C Chang, CE Weeden, GL Kelly, A Strasser
Cell Death and Differentiation, 2022-03-09;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Colorectal Cancer-Associated Immune Exhaustion Involves T and B Lymphocytes and Conventional NK Cells and Correlates With a Shorter Overall Survival
Authors: Carlo Sorrentino, Luigi D’Antonio, Cristiano Fieni, Stefania Livia Ciummo, Emma Di Carlo
Frontiers in Immunology
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Peripheral blood and tissue assessment highlights differential tumor-circulatory gradients of IL2 and MIF with prognostic significance in resectable pancreatic ductal adenocarcinoma
Authors: Azaz Ahmed, Sophia Köhler, Rosa Klotz, Nathalia Giese, Felix Lasitschka, Thilo Hackert et al.
OncoImmunology
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Association Between SARS-CoV-2 Infection and Immune-Mediated Myopathy in Patients Who Have Died
Authors: Tom Aschman, Julia Schneider, Selina Greuel, Jenny Meinhardt, Simon Streit, Hans-Hilmar Goebel et al.
JAMA Neurology
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Natural killer cells and cytotoxic T lymphocytes are required to clear solid tumor in a patient-derived xenograft
Authors: Duy Tri Le, Tridu R. Huynh, Bryan Burt, George Van Buren, Shawn A. Abeynaike, Cristina Zalfa et al.
JCI Insight
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Wilms' Tumor Primary Cells Display Potent Immunoregulatory Properties on NK Cells and Macrophages
Authors: PF Fiore, P Vacca, N Tumino, F Besi, A Pelosi, E Munari, M Marconi, I Caruana, V Pistoia, L Moretta, B Azzarone
Cancers, 2021-01-09;13(2):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Neuroblast senescence in the aged brain augments natural killer cell cytotoxicity leading to impaired neurogenesis and cognition
Authors: WN Jin, K Shi, W He, JH Sun, L Van Kaer, FD Shi, Q Liu
Nature Neuroscience, 2020-11-30;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Cellular and gene signatures of tumor-infiltrating dendritic cells and natural-killer cells predict prognosis of neuroblastoma
Authors: O Melaiu, M Chierici, V Lucarini, G Jurman, LA Conti, R De Vito, R Boldrini, L Cifaldi, A Castellano, C Furlanello, V Barnaba, F Locatelli, D Fruci
Nat Commun, 2020-11-25;11(1):5992.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors
Authors: JW Choi, S Lim, JH Kang, SH Hwang, KC Hwang, SW Kim, S Lee
Molecules, 2020-11-09;25(21):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Trastuzumab upregulates programmed death ligand-1 expression through interaction with NK cells in gastric cancer
Authors: K Yamashita, M Iwatsuki, N Yasuda-Yos, T Morinaga, Y Nakao, K Harada, K Eto, J Kurashige, Y Hiyoshi, T Ishimoto, Y Nagai, S Iwagami, Y Baba, Y Miyamoto, N Yoshida, JA Ajani, H Baba
Br J Cancer, 2020-10-26;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Chronic Inflammation Permanently Reshapes Tissue-Resident Immunity in Celiac Disease
Authors: Toufic Mayassi, Kristin Ladell, Herman Gudjonson, James E. McLaren, Dustin G. Shaw, Mai T. Tran et al.
Cell
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Mutated Von Hippel-Lindau-renal cell carcinoma (RCC) promotes patients specific natural killer (NK) cytotoxicity
Authors: AM Trotta, S Santagata, S Zanotta, C D'Alterio, M Napolitano, G Rea, R Camerlingo, F Esposito, E Lamantia, A Anniciello, G Botti, N Longo, G Botti, S Pignata, S Perdonà, S Scala
J. Exp. Clin. Cancer Res., 2018-12-04;37(1):297.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Targeted therapies for targeted populations: Anti-EGFR treatment for EGFR amplified gastroesophageal adenocarcinoma
Authors: SB Maron, L Alpert, HA Kwak, S Lomnicki, L Chase, D Xu, E O'Day, RJ Nagy, RB Lanman, F Cecchi, T Hembrough, A Schrock, J Hart, SY Xiao, N Setia, DVT Catenacci
Cancer Discov, 2018-02-15;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Infiltration and persistence of lymphocytes during late-stage cerebral ischemia in middle cerebral artery occlusion and photothrombotic stroke models
Authors: Y Feng, S Liao, C Wei, D Jia, K Wood, Q Liu, X Wang, FD Shi, WN Jin
J Neuroinflammation, 2017-12-15;14(1):248.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Low-risk population among patients with tumor-node-metastasis stage III/IV oral squamous cell carcinoma
Authors: Tohru Ikeda, Sachiko Seki, Mutsunori Fujiwara, Masaaki Matsuura, Yuu Ozaki-Honda, Shuichi Fujita et al.
Oncology Letters
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Activated hepatic stellate cells impair NK cell anti-fibrosis capacity through a TGF-?-dependent emperipolesis in HBV cirrhotic patients
Authors: J Shi, J Zhao, X Zhang, Y Cheng, J Hu, Y Li, X Zhao, Q Shang, Y Sun, B Tu, L Shi, B Gao, FS Wang, Z Zhang
Sci Rep, 2017-03-14;7(0):44544.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome
J Immunol, 2016-08-10;197(5):1621-30.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Natural Killer-like B Cells Prime Innate Lymphocytes against Microbial Infection.
Authors: Wang S, Xia P, Chen Y, Huang G, Xiong Z, Liu J, Li C, Ye B, Du Y, Fan Z
Immunity, 2016-07-12;45(1):131-44.
Species: Human, Mouse
Sample Types: Whole Tissue
Applications: IHC -
NKp30 isoforms and NKp46 transcripts in metastatic melanoma patients: Unique NKp30 pattern in rare melanoma patients with favorable evolution
Authors: M Messaouden, G Fregni, D Enot, N Jacquelot, E Neves, N Germaud, HJ Garchon, W Boukouaci, R Tamouza, J Chanal, MF Avril, A Toubert, L Zitvogel, S Rusakiewic, A Caignard
Oncoimmunology, 2016-03-10;5(12):e1154251.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Human liver-resident CD56(bright)/CD16(neg) NK cells are retained within hepatic sinusoids via the engagement of CCR5 and CXCR6 pathways.
Authors: Hudspeth K, Donadon M, Cimino M, Pontarini E, Tentorio P, Preti M, Hong M, Bertoletti A, Bicciato S, Invernizzi P, Lugli E, Torzilli G, Gershwin M, Mavilio D
J Autoimmun, 2015-08-30;66(0):40-50.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Characterization of the Microenvironment in Positive and Negative Sentinel Lymph Nodes from Melanoma Patients.
Authors: Messaoudene M, Perier A, Fregni G, Neves E, Zitvogel L, Cremer I, Chanal J, Sastre-Garau X, Deschamps L, Marinho E, Larousserie F, Maubec E, Avril M, Caignard A
PLoS ONE, 2015-07-28;10(7):e0133363.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
A novel tetravalent bispecific TandAb (CD30/CD16A) efficiently recruits NK cells for the lysis of CD30+ tumor cells.
Authors: Reusch U, Burkhardt C, Fucek I, Le Gall F, Le Gall M, Hoffmann K, Knackmuss S, Kiprijanov S, Little M, Zhukovsky E
MAbs, 2014-03-26;6(3):728-39.
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay -
CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph.
Authors: Carrega, Paolo, Bonaccorsi, Irene, Di Carlo, Emma, Morandi, Barbara, Paul, Petra, Rizzello, Valeria, Cipollone, Giuseppe, Navarra, Giuseppe, Mingari, Maria Cr, Moretta, Lorenzo, Ferlazzo, Guido
J Immunol, 2014-03-19;192(8):3805-15.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Mature cytotoxic CD56(bright)/CD16(+) natural killer cells can infiltrate lymph nodes adjacent to metastatic melanoma.
Cancer Res, 2013-11-13;74(0):81.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Natural killer cells are characterized by the concomitantly increased interferon-gamma and cytotoxicity in acute resolved hepatitis B patients.
Authors: Zhao J, Li Y, Jin L, Zhang S, Fan R, Sun Y, Zhou C, Shang Q, Li W, Zhang Z, Wang F
PLoS ONE, 2012-11-01;7(11):e49135.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Sequential metastases of colorectal cancer: Immunophenotypes and spatial distributions of infiltrating immune cells in relation to time and treatments.
Authors: Keim S, Zoernig I, Spille A, Lahrmann B, Brand K, Herpel E, Grabe N, Jager D, Halama N
Oncoimmunology, 2012-08-01;1(5):593-599.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Dimerization of NKp46 Receptor Is Essential for NKp46-Mediated Lysis: Characterization of the Dimerization Site by Epitope Mapping
Authors: Michal Jaron-Mendelson, Rami Yossef, Michael Y. Appel, Alon Zilka, Uzi Hadad, Fabian Afergan et al.
The Journal of Immunology
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Innate immunity in multiple sclerosis white matter lesions: expression of natural cytotoxicity triggering receptor 1 (NCR1).
Authors: Durrenberger PF, Ettorre A, Kamel F
J Neuroinflammation, 2012-01-02;9(0):1.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-Fr -
NKp46 identifies an NKT cell subset susceptible to leukemic transformation in mouse and human.
Authors: Yu J, Mitsui T, Wei M, Mao H, Butchar JP, Shah MV, Zhang J, Mishra A, Alvarez-Breckenridge C, Liu X, Liu S, Yokohama A, Trotta R, Marcucci G, Benson DM, Loughran TP, Tridandapani S, Caligiuri MA
J. Clin. Invest., 2011-04-01;121(4):1456-70.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation.
Authors: Dulphy N, Haas P, Busson M, Belhadj S, Peffault de Latour R, Robin M, Carmagnat M, Loiseau P, Tamouza R, Scieux C, Rabian C, Di Santo JP, Charron D, Janin A, Socie G, Toubert A
J. Immunol., 2008-08-01;181(3):2227-37.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
Authors: Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD, Barlogie B, van Rhee F
Blood, 2007-10-18;111(3):1309-17.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
A novel binding assay to assess specificity of monoclonal antibodies.
Authors: Warren HS, Warren</LastName><ForeNam HS</Initia, Bettadapura J
J. Immunol. Methods, 2005-08-15;305(1):33-8.
Species: Human
Sample Types: Complex Sample Type
Applications: Epitope Mapping -
Case Report: A Peculiar Case of Inflammatory Colitis After SARS-CoV-2 Infection
Authors: Rutigliani M, Bozzo M, Barberis A et al.
Frontiers in immunology
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An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer
Authors: Johnson BE, Creason AL, Stommel JM et al.
Cell reports. Medicine
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Cytotoxic effects of ex vivo-expanded natural killer cell-enriched lymphocytes (MYJ1633) against liver cancer
Authors: Jung-Won Choi, Eui Soo Lee, Se Young Kim, Su Il Park, Sena Oh, Jung Hwa Kang et al.
BMC Cancer
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