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Human NgR3/NgRH2 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF2920V Datasheet
Catalog # Availability Size / Price Qty
AF2920V-100UG
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Human NgR3/NgRH2 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human NgR3/NgRH2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross‑reactivity with recombinant human (rh) NgRH1 is observed and less than 1% cross-reactivity with rhNgR is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human NgR3/NgRH2
Cys25-Ala419
Accession # Q86UN2
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: NgR3/NgRH2

NgR3, also known as Nogo 66 receptor homolog 2 (NgRH2) and reticulon 4 receptor-like 1 (RTN4RL1), is a 60 kDa GPI-linked glycoprotein belonging to the family of leucine-rich repeat (LRR) proteins. It contains eight central LRRs that are flanked by a LRR N-terminal subdomain and a cysteine-rich C-terminal subdomain. Human NgR3 shares approximately 89% amino acid sequence homology with the mouse or rat protein.

Long Name
Nogo-66 Receptor-related Protein 3/Nogo-66 Receptor Homolog 2/Reticulon-4 Receptor-like 1
Entrez Gene IDs
146760 (Human); 237847 (Mouse)
Alternate Names
DKFZp547J144; NgR3; NgRH2; NGRL2; nogo receptor-like 2; Nogo-66 receptor homolog 2; Nogo-66 receptor-related protein 3; reticulon 4 receptor-like 1; reticulon-4 receptor-like 1; RTN4RL1

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