Human Nestin Fluorescein-conjugated Antibody Summary
Accession # P48681
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Nestin in NTera‑2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human Nestin Fluorescein-conjugated Monoclonal Antibody (Catalog # IC1259F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
Background: Nestin
Nestin is a class VI intermediate filament protein that is expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells. Its transient expression is a critical step in both the neural differentiation pathway and that of CNS glia. Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors and hematopoietic progenitors. Full-length human Nestin shares 51% amino acid sequence identity to mouse Nestin.
Product Datasheets
Citations for Human Nestin Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Neural stem cell transplantation in patients with progressive multiple sclerosis: an open-label, phase 1 study
Authors: A Genchi, E Brambilla, F Sangalli, M Radaelli, M Bacigalupp, R Furlan, A Andolfo, D Drago, C Magagnotti, GM Scotti, R Greco, P Vezzulli, L Ottoboni, M Bonopane, D Capilupo, F Ruffini, D Belotti, B Cabiati, S Cesana, G Matera, L Leocani, V Martinelli, L Moiola, L Vago, P Panina-Bor, A Falini, F Ciceri, A Uglietti, MP Sormani, G Comi, MA Battaglia, MA Rocca, L Storelli, E Pagani, G Gaipa, G Martino
Nature Medicine, 2023-01-09;29(1):75-85.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium
Authors: S Mount, P Kanda, S Parent, S Khan, C Michie, L Davila, V Chan, RA Davies, H Haddad, D Courtman, DJ Stewart, DR Davis
Stem Cell Res Ther, 2019-11-04;10(1):316.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Expression of a SOX1 overlapping transcript in neural differentiation and cancer models
Authors: Azaz Ahmad, Stephanie Strohbuecker, Cristina Tufarelli, Virginie Sottile
Cellular and Molecular Life Sciences
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Non integrative strategy decreases chromosome instability and improves endogenous pluripotency genes reactivation in porcine induced pluripotent-like stem cells
Authors: Annabelle Congras
Sci Rep, 2016-06-01;6(0):27059.
Species: Porcine
Sample Types: Whole Cells
Applications: Flow Cytometry -
Modeling Smith-Lemli-Opitz syndrome with induced pluripotent stem cells reveals a causal role for Wnt/?-catenin defects in neuronal cholesterol synthesis phenotypes
Nat. Med., 2016-03-21;22(4):388-96.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Arsenic trioxide-mediated Notch pathway inhibition depletes the cancer stem-like cell population in gliomas.
Authors: Zhen Y, Zhao S, Li Q
Cancer Lett., 2009-12-04;292(1):64-72.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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