Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody
Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody Summary
Leu189-Leu741
Accession # Q09328
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human N‑Acetylglucosaminyltransferase V/MGAT5 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human N-Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Catalog # MAB5469) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for N-Acetylglucosaminyltransferase V/MGAT5 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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N‑Acetylglucosaminyltransferase V/MGAT5 in MCF‑7 Human Cell Line. N-Acetylglucosaminyltransferase V/MGAT5 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human N-Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Catalog # MAB5469) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: N-Acetylglucosaminyltransferase V/MGAT5
N-Acetylglucosaminyltransferase V (GnT-V), also known as mannosylglycoprotein N-acetyl-glucosaminyltransferase 5 (MGAT5), adds an N-acetylglucosamine to the alpha 1-6-linked core mannose of an N-linked oligosaccharide in the Golgi apparatus (1). This reaction is the committing step for the biosynthesis of beta 1-6GlcNAc-branched arm in N-glycans. The degree of N-glycan branching has been shown to regulate cell proliferation and differentiation (2). An increase in the GnT-V activity and its glycan products is also known to positively correlate with the progression of invasive malignancies (3, 4). For example, ectopic expression of GnT-V in epithelial cells results in morphological transformation and tumor growth in mice and overexpression in carcinoma cells has been shown to induce metastatic spread (3-5).
- Saito, H. et al. (1994) Biochem. Biophys. Res. Commun. 198:318.
- Lau, K.S. et al. (2007) Cell 198:123.
- Dennis, J.W. et al. (2002) Biochim. Biophys. Acta 1573:414.
- Granovsky, M. et al. (2008) Nat. Med. 7:1.
- Kim, Y.S. et al. (2008) Mol. Cell. Proteomics 7:1.
Product Datasheets
Citations for Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Shedding of N-acetylglucosaminyltransferase-V is regulated by maturity of cellular N-glycan
Authors: Tetsuya Hirata, Misaki Takata, Yuko Tokoro, Miyako Nakano, Yasuhiko Kizuka
Communications Biology
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N-Acetylglucosaminyltransferase-V requires a specific non-catalytic luminal domain for its activity toward glycoprotein substrates
Authors: RF Osuka, T Hirata, M Nagae, M Nakano, H Shibata, R Okamoto, Y Kizuka
The Journal of Biological Chemistry, 2022-01-30;0(0):101666.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Epithelial-to-Mesenchymal Transition of RPE Cells In Vitro Confers Increased beta1,6-N-Glycosylation and Increased Susceptibility to Galectin-3 Binding.
Authors: Priglinger C, Obermann J, Szober C, Merl-Pham J, Ohmayer U, Behler J, Gruhn F, Kreutzer T, Wertheimer C, Geerlof A, Priglinger S, Hauck S
PLoS ONE, 2016-01-13;11(1):e0146887.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Shedding of glycan-modifying enzymes by signal peptide peptidase-like 3 (SPPL3) regulates cellular N-glycosylation.
Authors: Voss M, Kunzel U, Higel F, Kuhn P, Colombo A, Fukumori A, Haug-Kroper M, Klier B, Grammer G, Seidl A, Schroder B, Obst R, Steiner H, Lichtenthaler S, Haass C, Fluhrer R
EMBO J, 2014-10-29;33(24):2890-905.
Species: Human, Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
N-acetylglucosaminyltransferase-V (GnT-V)-enriched small extracellular vesicles mediate N-glycan remodeling in recipient cells
Authors: Tetsuya Hirata, Yoichiro Harada, Koichiro M. Hirosawa, Yuko Tokoro, Kenichi G.N. Suzuki, Yasuhiko Kizuka
iScience
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