Human MSPR/Ron PE-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: MSPR/Ron

RON (Recepteur d'Origine Nantais [Receptor originating (or discovered) in Nantes (France)]; also known as MSP-R, PTK8 and MST1R) is a 180-190 kDa member of the tyrosine kinase family, protein kinase superfamily of molecules. Human mature RON is a type I transmembrane heterodimeric glycoprotein that arises from the proteolytic cleavage of a single chain proform 1376 amino acids (aa) in length. Posttranslational processing generates a 35-40 kDa a-chain (aa 25-304) coupled to a 145-150 kDa b-chain (aa 310-1400). The chains are disulfide-linked, and the transmembrane segment appears over aa 958-978. Multiple cell types are known to express RON, and these include osteoclasts, TrkA+ dorsal root ganglia neurons, macrophages, keratinized and nonkeratinized stratified squamous epithelium, intestinal Paneth cells, renal proximal tubule and mesangial cells, monocytes, neutrophils and mammary epithelium. On the cell surface, RON serves as a receptor for MSP, a 78-80 kDa soluble molecule related to HGF. Ligand binding initiates homodimerization and receptor signaling. In addition, RON is also known to heterodimerize with multiple integral membrane proteins, either individually or as part of a complex. Proteins identified to date include b1 integrins, c-MET/HGFR, E-Cadherin, the bc-chain for IL-3, EPO-R, EGFR, IGF-IR, Plexins B1 and B3, and CD44v6. RON may also be found intracellularly, where it interacts with either the androgen receptor or EGFR in the cytoplasm, or in the nucleus, where it acts as a transcription factor coupled to HIF-1a and regulates c-Jun, Bcl-2 and c-FLIP expression. There are about a dozen alternative splice forms for RON, some soluble, while others are transmembrane, and either constitutively active or biochemically inert. They hold the potential to interact with full-length RON and impact its signaling capabilities. Reflecting the diverse nature of RON-expressing cells, RON has been found to generate multiple outcomes, including the promotion of epithelial-to-mesenchymal transitions, the maintenance of M2 macrophage phenotypes, and the initiation of keratinocyte proliferation and migration. Over aa 25-956, human and mouse RON share 76% aa sequence identity.

Product Datasheets

Related Research Areas

• Cancer Biomarkers
• Epithelial Cell Markers and Intracellular Molecules
• Receptor Tyrosine Kinases (RTKs) in the Akt Pathway
• Surface Ectodermal Cells