Human/Mouse/Rat TRAF-2 Antibody Summary
Met1-Leu501
Accession # Q12933
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat TRAF‑2 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, SW3T3 mouse contact inhibited fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.25 µg/mL Mouse Anti-Human/Mouse/Rat TRAF-2 Monoclonal Antibody (Catalog # MAB3277) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human TRAF-1, -2, -3, -4, -5, and -6 (2 ng/lane) were included. A specific band for TRAF-2 was detected at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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TRAF‑2 in HeLa Human Cell Line. TRAF-2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat TRAF-2 Monoclonal Antibody (Catalog # MAB3277) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Western Blot Shows Human TRAF‑2 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and TRAF-2 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat TRAF-2 Monoclonal Antibody (Catalog # MAB3277) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TRAF-2 at approximately 62 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAF-2
Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL-1 receptor family members and mediate cellular signaling. Six members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs 2‑6 have N-terminal RING and zinc finger domains that are involved in signaling downstream events. TRAF-2, also known as TNF Receptor-Associated Protein (TRAP), is a 501 amino acid, 56 kDa protein which interacts with the cytoplasmic domain of TNFR1, TNFR2 and CD40 to mediate the activation of NF-kappa-B and AP-1 family of transcription factors. TRAF-2 can self-associate as well as form heterodimers with TRAF-1.
Product Datasheets
Citations for Human/Mouse/Rat TRAF-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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The CD40 agonist HERA-CD40L results in enhanced activation of antigen presenting cells, promoting an anti-tumor effect alone and in combination with radiotherapy
Authors: Jamie Frankish, Debayan Mukherjee, Erminia Romano, Katharina Billian-Frey, Matthias Schröder, Karl Heinonen et al.
Frontiers in Immunology
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Molecular and cellular evidence of a direct interaction between the TRAF2 C-terminal domain and ganglioside GM1
Authors: De Luca, A;Faienza, F;Fulci, C;Nicolai, E;Calligari, P;Palumbo, C;Caccuri, AM;
The international journal of biochemistry & cell biology
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
NEDD4 ubiquitinates TRAF3 to promote CD40-mediated AKT activation.
Authors: Fang, Di-Feng, He, Kun, Wang, Na, Sang, Zhi-Hong, Qiu, Xin, Xu, Guang, Jian, Zhao, Liang, Bing, Li, Tao, Li, Hui-Yan, Li, Ai-Ling, Zhou, Tao, Gong, Wei-Li, Yang, Baoli, Karin, Michael, Zhang, Xue-Min, Li, Wei-Hua
Nat Commun, 2014-07-29;5(0):4513.
Species: Human, Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Zinc-finger protein 91 plays a key role in LIGHT-induced activation of non-canonical NF-kappaB pathway.
Authors: Jin HR, Jin X, Lee JJ
Biochem. Biophys. Res. Commun., 2010-09-06;400(4):581-6.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation.
Authors: Kolliputi N, Waxman AB
Am. J. Respir. Cell Mol. Biol., 2008-09-05;40(3):314-24.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The function of TRADD in signaling through tumor necrosis factor receptor 1 and TRIF-dependent Toll-like receptors.
Authors: Pobezinskaya YL, Kim YS, Choksi S, Morgan MJ, Li T, Liu C, Liu Z
Nat. Immunol., 2008-07-20;9(9):1047-54.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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