Human/Mouse/Rat STAT3 PE-conjugated Antibody Summary
Met1-Asn175
Accession # P40763
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of STAT3 in HeLa Human Cell Line by Flow Cytometry. HeLa human cell line was stained with Mouse Anti-Human/Mouse/Rat STAT3 PE-conjugated Monoclonal Antibody (Catalog # IC1799P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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STAT3 Specificity is Shown by Flow Cytometry in Knockout Cell Line. STAT3 knockout HeLa human cervical epithelial cell line was stained with PE-conjugated Mouse Anti-Human/Mouse STAT3 Monoclonal Antibody (Catalog # IC1799P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). No staining in the STAT3 knockout HeLa cell line was observed. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: STAT3
Signal Transducer and Activator of Transcription (STAT) proteins are transcription factors activated in response to cytokine, growth factor, or hormone receptor signaling. STAT3 is an 89-92 kDa, and most widely expressed, member of the STAT-family of molecules. STAT3 is considered a direct transcription factor; that is it does not act in an indirect way such as epigenetically through the regulation of DNA methylation. Activation/phosphorylation of STAT3 results in homodimerization or heterodimerization with STAT1, whereupon it enters the nucleus to principally promote cell cycle progression. The propensity of STAT3 and STAT5 toward cell division is of particular interest in oncology. Over amino acids 1-175 of STAT3 that include a protein-protein interaction domain and an NLS, human, rat and mouse show 100% amino acid sequence identity.
Product Datasheets
Citations for Human/Mouse/Rat STAT3 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Antimicrobial Peptide LCN2 Inhibited Uropathogenic Escherichia coli Infection in Bladder Cells in a High-Glucose Environment through JAK/STAT Signaling Pathway
Authors: PC Chen, CH Ho, CK Fan, SP Liu, PC Cheng
International Journal of Molecular Sciences, 2022-12-12;23(24):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Opposing Effects of Chelidonine on Tyrosine and Serine Phosphorylation of STAT3 in Human Uveal Melanoma Cells
Authors: István Csomós, Péter Nagy, Csenge Filep, István Rebenku, Enikő Nizsalóczki, Tamás Kovács et al.
International Journal of Molecular Sciences
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