Human/Mouse/Rat ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody
Human/Mouse/Rat ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody Summary
Pro52-Gln350
Accession # NP_008858
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat ST3 beta -Gal alpha -2,3-Sialyltransferase 2/ST3GAL2 by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human ST3 beta -Gal a-2,3-Sialyltransferase 2/ST3GAL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7275) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST3 beta -Gal a-2,3-Sialyltransferase 2/ST3GAL2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2
Sialyltransferases add sialic acid to glycoproteins or glycosphingolipids and play important roles in many biological processes including immune recognition, pathogen infection, and cell adhesion (1). Similar to ST3GAL1 (2), ST3GAL2 is a type II membrane protein localized in the trans-Golgi network that can transfer sialic acid to the Gal beta 1-3GalNAc structure to form NeuAc alpha 2-3Gal beta 1-3GalNAc found in terminal carbohydrate groups of particular glycoproteins, oligosaccharides and glycolipids (3). Unlike the ubiquitously expressed ST3GAL1 (4), ST3GAL2 is mainly expressed in heart, liver, skeletal muscle and various lymphoid tissues but not in brain and kidney (3). ST3GAL2 is responsible for the synthesis of monosialosyl globopentaosylceramide (MSGb5), also known as stage-specific embryonic antigen-4 (SSEA4) (5), a cell surface marker for testicular germ cell carcinoma, renal cell carcinoma, and mesenchymal stem cells (6). The enzymatic activity of recombinant human ST3GAL2 was determined using a phosphatase-coupled glycosyltransferase assay (7). Over aa 52-350 human ST3GAL2 share 94% aa sequence identity with mouse and rat.
- Varki, A. (1999) Glycobiology 2:25.
- Jeanneau, C. et al. (2004) J. Biol. Chem. 279:13461.
- Giordanengo, V. et al. (1997) Eur. J. Biochem. 247:558.
- Kitagawa, H. and Paulson, J.C. (1994) J. Biol. Chem. 269:17872.
- Saito, S. et al. (2003) J. Biol. Chem. 278:26474.
- Suila, H. et al. (2011) J. Mol. Cell Biol. 3:99.
- Wu, Z.L. et al. (2011) Glycobiology 21:727.
Product Datasheets
Citation for Human/Mouse/Rat ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Sialidase inhibitors attenuate pulmonary fibrosis in a mouse model
Authors: TR Karhadkar, D Pilling, N Cox, RH Gomer
Sci Rep, 2017-11-08;7(1):15069.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
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