Human/Mouse/Rat SOD2/Mn-SOD Antibody Summary
Lys25-Lys222
Accession # P04179
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat SOD2/Mn-SOD by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOD2/Mn-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3419) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOD2/Mn-SOD at approximately 23 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
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Detection of Human and Mouse SOD2/Mn‑SOD by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for SOD2/Mn-SOD at approximately 28 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat SOD2/Mn-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3419) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Mouse SOD2/Mn-SOD by Western Blot CD138low cells have altered mitochondrial properties that can be utilized to induce oxidative stress by 10-TPP treatment E. HMCLs transduced with Ad-CMV or Ad-MnSOD, treated with 10-TPP,&MitoSOX oxidation was analyzed by flow cytometry. Data is presented as fold change normalized to control cells expressing Ad-CMV. Antimycin A treatment was used as a positive control. *p < 0.01 vs. control cells (Ad-CMV or Ad-MnSOD), #p < 0.05 vs. 10-TPP treated Ad-CMV cells. Representative WB of HMCLs transduced with Ad-CMV or Ad-MnSOD (MOI = 50), whole-cell extract was made at 48 h&probed with an antibody against MnSOD (24 kDa) or beta -actin. The quantification of MnSOD after normalization to untreated control is shown below each band. F. Structure of 10-TPVP; representative confocal images of MM.1S&OPM-2 cells stained with MitoTracker red&10-TPVP. The bright field view&merge image of MitoTracker Red&10-TPVP are also shown. For panels B-E, bars represent mean of three independent runs ± SEM. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27902770), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOD2/Mn-SOD
Superoxide Dismutases (SODs), originally identified as Indophenoloxidases (IPOs), are enzymes that catalyze the conversion of naturally occurring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutases 2 (SOD2, also known as Manganese (Mn) SOD, mitochondrial SOD and IPO-B) is an intramitochondrial 23 kDa protein that associates as a homotetramer. Each SOD2 monomer binds one Mn2+ ion. Oxidative stress has been implicated in many diseases and the chief source of reactive oxygen species within the cell is the mitochondrion. SOD2 is a free radical scavenging enzyme that protects against damage from superoxide produced as a byproduct of oxidative phosphorylation and protects the integrity of mitochondrial enzymes susceptible to inactivation by superoxide. Over aa 25‑222, human SOD2 shows 94% and 93% identity to mouse and rat SOD2.
Product Datasheets
Citations for Human/Mouse/Rat SOD2/Mn-SOD Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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TLR4-Mediated Inflammatory Responses Regulate Exercise-Induced Molecular Adaptations in Mouse Skeletal Muscle
Authors: H Fujiyoshi, T Egawa, E Kurogi, T Yokokawa, K Kido, T Hayashi
International Journal of Molecular Sciences, 2022-02-07;23(3):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Mitochondrial-Targeted Decyl-Triphenylphosphonium Enhances 2-Deoxy-D-Glucose Mediated Oxidative Stress and Clonogenic Killing of Multiple Myeloma Cells
PLoS ONE, 2016-11-30;11(11):e0167323.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Initiation of 3,3‐dimethyl‐1‐butanol at midlife prevents endothelial dysfunction and attenuates in vivo aortic stiffening with ageing in mice
Authors: Abigail G. Casso, Nicholas S. VanDongen, Rachel A. Gioscia‐Ryan, Zachary S. Clayton, Nathan T. Greenberg, Brian P. Ziemba et al.
The Journal of Physiology
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Rejuvenation of mesenchymal stem cells by extracellular vesicles inhibits the elevation of reactive oxygen species
Authors: Vuong Cat Khanh, Toshiharu Yamashita, Kinuko Ohneda, Chiho Tokunaga, Hideyuki Kato, Motoo Osaka et al.
Scientific Reports
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