Human/Mouse/Rat Properdin Antibody

Catalog #: AF8216
1 Citation 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
AF8216
AF8216-SP

Detection of Human, Mouse, and Rat Properdin by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Properdin Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat Properdin in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Human embryonic kidney cell line HEK293-derived human Properdin
Asp28-Leu469
Accession # P27918

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Properdin by Western Blot. Western blot shows lysates of human serum, mouse serum, and rat serum. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Properdin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8216) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Properdin at approximately 53-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Properdin

Human Properdin, also known as Complement factor P (CFP), is a 53 kDa secreted glycoprotein that exists in plasma as dimers, trimers or tetramers. It a positive regulator of the alternate pathway of complement. Properdin binds to and stabilizes the C3- and C5-convertase enzyme complexes and is thought to connect humoral and cellular immunity. Properdin released from activated neutrophils may play a major role in pattern recognition, whereas plasma properdin may primarily stabilize convertase. Properdin deficiency results in higher susceptibility to bacterial infections. Full-length human Properdin shares 77 % sequence identity with mouse Properdin.

Entrez Gene IDs

5199 (Human); 18636 (Mouse); 299314 (Rat)

Alternate Names

BFD; CFP; Complement Factor P; complement factor properdin; PFC; PFCcomplement; PFD; Properdin

Product Datasheets

Product Datasheet

Related Research Areas

Complement Pathway

Citation for Human/Mouse/Rat Properdin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1