Human/Mouse/Rat p70 S6 Kinase Alexa Fluor® 488-conjugated Antibody

Catalog #: IC8962G Datasheet

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IC8962G-025

Detection of p70 S6 Kinase in HeLa Human Cell Line by Flow Cytometry.

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Human/Mouse/Rat p70 S6 Kinase Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat p70 S6 Kinase and p85 S6 Kinase in Western blots. Reactivity with beta isoforms of p70 S6 Kinase is unknown.

Source

Monoclonal Mouse IgG₁ Clone # 215247

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human p70 S6 Kinase
Accession # M60725

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of p70 S6 Kinase antibody in HeLa Human Cell Line antibody by Flow Cytometry.

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Detection of p70 S6 Kinase in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat p70 S6 Kinase Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC8962G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: p70 S6 Kinase

p70 S6 Kinase (p70S6K) is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues. p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K activity undergoes changes during the cell cycle and increases 20‑fold in G1 cells released from G0. p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well asT389, a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK1) (1-3). Over amino acids (aa) 1-502, human and mouse share 99% aa sequence identity.

References

Ferrari, S. et al. (1994) Crit. Rev. Biochem. Mol. Biol. 29:385.
Edelmann, H.M. et al. (1996) J. Biol. Chem. 271:963.
Fenton, T.R. and I.T. Gout (2011) Int. J. Biochem. Cell Biol. 43:47.

Entrez Gene IDs

6198 (Human); 72508 (Mouse); 83840 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.1; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6 Kinase; p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1; p70 S6 kinase, alpha 2; p70 S6KA; p70 S6K-alpha; p70(S6K)-alpha; p70-alpha; p70-S6K; P70S6K1; PS6K; ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kD, polypeptide 1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; RPS6KB1; S6K; S6K1; S6K1p70-S6K 1; S6K-beta-1; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A; STK14A