Human/Mouse/Rat p53 HRP-conjugated Antibody

Detection of Human p53 by Western Blot. Western blot shows lysates of CEM human T‑lymphoblastoid cell line and MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 10 Gy ionizing radiation (IR) for 1 hour. PVDF membrane was probed with 1:5000 dilution of Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # HAF1355). A specific band was detected for p53 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human p53 by Western Blot Western blotting confirmed doxorubicin-induced increase in expression of p53 and DRs.a Immunoblot and b densitometric quantification demonstrate after exposing cardiomyocytes to 150 nM or 500 nM of doxorubicin for 48 h the phosphorylation of p53 and total expression of p53, TNFR1, DR4, DR5, and Fas increase. TNFR1 protein expression increased only after exposure to 500 nM of doxorubicin. The protein expression of p53 targets, DR4 and DR5, decrease after a 7-day washout period. Fas remains slightly upregulated after 7-day washout Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31231550), licensed under a CC-BY license. Not internally tested by R&D Systems.